Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology
| Autor: | Marin Poljak, Samantha Walker, Elisa Mokany, Suzanne M. Garland, Christopher K Fairley, Lit Yeen Tan, Sepehr N. Tabrizi, Catriona S. Bradshaw, Melanie Bissessor, Alison V. Todd, Jimmy Twin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male DNA Mutational Analysis Gene Identification and Analysis Cervicitis lcsh:Medicine Mycoplasma genitalium Drug resistance medicine.disease_cause Azithromycin Pathology and Laboratory Medicine urologic and male genital diseases Biochemistry Multiplexing 0302 clinical medicine Mycoplasma Pelvic inflammatory disease Medicine and Health Sciences Multiplex 030212 general & internal medicine lcsh:Science Multidisciplinary female genital diseases and pregnancy complications Bacterial Pathogens Anti-Bacterial Agents Bacterial Typing Techniques Nucleic acids Ribosomal RNA Medical Microbiology Neisseria Gonorrhoeae Telecommunications Engineering and Technology Female Macrolides Pathogens Neisseria medicine.drug Research Article Cell biology Cellular structures and organelles 030106 microbiology Microbial Sensitivity Tests Biology Microbiology Sensitivity and Specificity 03 medical and health sciences Drug Resistance Bacterial medicine Genetics Humans Urethritis Mycoplasma Infections Non-coding RNA Mutation Detection Microbial Pathogens Bacteria lcsh:R Organisms Biology and Life Sciences Reproducibility of Results DNA medicine.disease biology.organism_classification bacterial infections and mycoses Virology Mutation RNA lcsh:Q Ribosomes Multiplex Polymerase Chain Reaction Neisseria Meningitidis |
| Zdroj: | PLoS ONE, Vol 11, Iss 6, p e0156740 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Mycoplasma genitalium is a cause of non-gonoccocal urethritis (NGU) in men and cervicitis and pelvic inflammatory disease in women. Recent international data also indicated that the first line treatment, 1 gram stat azithromycin therapy, for M. genitalium is becoming less effective, with the corresponding emergence of macrolide resistant strains. Increasing failure rates of azithromycin for M. genitalium has significant implications for the presumptive treatment of NGU and international clinical treatment guidelines. Assays able to predict macrolide resistance along with detection of M. genitalium will be useful to enable appropriate selection of antimicrobials to which the organism is susceptible and facilitate high levels of rapid cure. One such assay recently developed is the MG 23S assay, which employs novel PlexZyme™ and PlexPrime™ technology. It is a multiplex assay for detection of M. genitalium and 5 mutations associated with macrolide resistance. The assay was evaluated in 400 samples from 254 (186 males and 68 females) consecutively infected participants, undergoing tests of cure. Using the MG 23S assay, 83% (331/440) of samples were positive, with 56% of positives carrying a macrolide resistance mutation. Comparison of the MG 23S assay to a reference qPCR method for M. genitalium detection and high resolution melt analysis (HRMA) and sequencing for detection of macrolide resistance mutations, resulted in a sensitivity and specificity for M. genitalium detection and for macrolide resistance of 99.1/98.5% and 97.4/100%, respectively. The MG 23S assay provides a considerable advantage in clinical settings through combined diagnosis and detection of macrolide resistance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|