Concentrations and molecular forms of human brain natriuretic peptide in plasma
| Autor: | Hisayuki Matsuo, T. Ogihara, Hitone Tateyama, Kei Sakai, Jun Hino, Naoto Minamino, Kenji Kangawa, Takazo Minamino |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Heart Diseases Heart disease medicine.drug_class Radioimmunoassay Biophysics Nerve Tissue Proteins Peptide hormone Biochemistry Chromatography Affinity Atrial natriuretic peptide Internal medicine Natriuretic Peptide Brain Blood plasma medicine Natriuretic peptide Humans cardiovascular diseases Molecular Biology Chromatography High Pressure Liquid Chemistry Cell Biology Human brain Brain natriuretic peptide medicine.disease Molecular Weight Endocrinology medicine.anatomical_structure cardiovascular system human activities hormones hormone substitutes and hormone antagonists circulatory and respiratory physiology |
| Zdroj: | Biochemical and Biophysical Research Communications. 185:760-767 |
| ISSN: | 0006-291X |
| Popis: | Using a highly sensitive radioimmunoassay (RIA) system for human brain natriuretic peptide (BNP), immunoreactive (ir-) human BNP was found to be present in plasma, in addition to heart and brain tissue. Plasma concentrations of ir-BNP were 0.17-0.74 fmol/ml (mean: 0.35 fmol/ml) in normal young men, being about 1/17 of the plasma concentration of human atrial natriuretic peptide (ANP). In patients with heart disease, plasma concentration of ir-BNP increased about 100-fold (5.00-177.37 fmol/ml), being nearly comparable to that of ir-ANP, even though ANP concentration also increased about 7-fold. Two molecular forms of ir-BNP in plasma were identified as BNP-32 and gamma-BNP (pro-BNP), which are also found in cardiac atrium. In normal human plasma, gamma-BNP is the predominant molecular form, while the main form in cardiac atrium is BNP-32. These results suggest that biosynthesis and secretion of BNP are augmented in heart disease and that human BNP has a unique processing and metabolic system distinct from that of ANP. |
| Databáze: | OpenAIRE |
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