Development and Validation of an Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Method for Quantitative Analysis of Platinum in Plasma, Urine, and Tissues
| Autor: | Shuang Cai, Eva Mohr, M. Laird Forrest, Wai Chee Forrest, Qiuhong Yang, Ti Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Accuracy and precision Bioanalysis chemistry.chemical_element Urine Sensitivity and Specificity Article Mass Spectrometry 03 medical and health sciences 0302 clinical medicine Drug Stability Animals Tissue Distribution Instrumentation Inductively coupled plasma mass spectrometry Spectroscopy Platinum Chromatography Chemistry Reproducibility of Results Plasma Quantitative determination Rats 030104 developmental biology 030220 oncology & carcinogenesis Linear Models Quantitative analysis (chemistry) |
| Zdroj: | Applied Spectroscopy. 70:1529-1536 |
| ISSN: | 1943-3530 0003-7028 |
| Popis: | Cisplatin, a platinum chemotherapeutic, is one of the most commonly used chemotherapeutic agents for many solid tumors. In this work, we developed and validated an inductively coupled plasma mass spectrometry (ICP-MS) method for quantitative determination of platinum levels in rat urine, plasma, and tissue matrices including liver, brain, lungs, kidney, muscle, heart, spleen, bladder, and lymph nodes. The tissues were processed using a microwave accelerated reaction system (MARS) system prior to analysis on an Agilent 7500 ICP-MS. According to the Food and Drug Administration guidance for industry, bioanalytical validation parameters of the method, such as selectivity, accuracy, precision, recovery, and stability were evaluated in rat biological samples. Our data suggested that the method was selective for platinum without interferences caused by other presenting elements, and the lower limit of quantification was 0.5 ppb. The accuracy and precision of the method were within 15% variation and the recoveries of platinum for all tissue matrices examined were determined to be 85–115% of the theoretical values. The stability of the platinum-containing solutions, including calibration standards, stock solutions, and processed samples in rat biological matrices was investigated. Results indicated that the samples were stable after three cycles of freeze–thaw and for up to three months. |
| Databáze: | OpenAIRE |
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