Impact of Pancreatic Rat Islet Density on Cell Survival during Hypoxia
| Autor: | J Magisson, A. Langlois, C. Peronet, Carole Mura, Cynthia Sookhareea, Michel Pinget, Nathalie Jeandidier, Elisa Maillard, A Rodriguez-Brotons, Séverine Sigrist, William Bietiger |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty endocrine system Time Factors endocrine system diseases Article Subject Cell Survival Endocrinology Diabetes and Metabolism Apoptosis Biology lcsh:Diseases of the endocrine glands. Clinical endocrinology Tissue Culture Techniques Islets of Langerhans 03 medical and health sciences Endocrinology Internal medicine Gene expression medicine Animals Viability assay Rats Wistar Regulation of gene expression geography lcsh:RC648-665 geography.geographical_feature_category Oxygenation Hypoxia-Inducible Factor 1 alpha Subunit Islet Cell Hypoxia Oxygen Transplantation Atmospheric Pressure 030104 developmental biology Gene Expression Regulation Cytokines Cytokine secretion Inflammation Mediators Research Article |
| Zdroj: | Journal of Diabetes Research, Vol 2016 (2016) Journal of Diabetes Research |
| ISSN: | 2314-6745 |
| DOI: | 10.1155/2016/3615286 |
| Popis: | In bioartificial pancreases (BP), the number of islets needed to restore normoglycaemia in the diabetic patient is critical. However, the confinement of a high quantity of islets in a limited space may impact islet survival, particularly in regard to the low oxygen partial pressure (PO2) in such environments. The aim of the present study was to evaluate the impact of islet number in a confined space under hypoxia on cell survival. Rat islets were seeded at three different concentrations (150, 300, and 600 Islet Equivalents (IEQ)/cm2) and cultured in normal atmospheric pressure (160 mmHg) as well as hypoxic conditions (15 mmHg) for 24 hours. Cell viability, function, hypoxia-induced changes in gene expression, and cytokine secretion were then assessed. Notably, hypoxia appeared to induce a decrease in viability and increasing islet density exacerbated the observed increase in cellular apoptosis as well as the loss of function. These changes were also associated with an increase in inflammatory gene transcription. Taken together, these data indicate that when a high number of islets are confined to a small space under hypoxia, cell viability and function are significantly impacted. Thus, in order to improve islet survival in this environment during transplantation, oxygenation is of critical importance. |
| Databáze: | OpenAIRE |
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