Viability of D283 medulloblastoma cells treated with a histone deacetylase inhibitor combined with bombesin receptor antagonists
| Autor: | Eduarda Chiesa Ghisleni, Lívia Fratini, André T Brunetto, Mariane da Cunha Jaeger, Lauro José Gregianin, Gilberto Schwartsmann, Rafael Roesler, Caroline Brunetto de Farias, Algemir Lunardi Brunetto |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cell Survival medicine.drug_class Antineoplastic Agents Apoptosis Biology Peptides Cyclic 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor medicine Gastrin-releasing peptide receptor Humans Epigenetics Medulloblastoma Analysis of Variance Dose-Response Relationship Drug Histone deacetylase inhibitor Bombesin Sodium butyrate General Medicine medicine.disease Peptide Fragments Bombesin receptor Histone Deacetylase Inhibitors Receptors Bombesin 030104 developmental biology chemistry Pediatrics Perinatology and Child Health Cancer research Neurology (clinical) Histone deacetylase 030217 neurology & neurosurgery |
| Zdroj: | Child's Nervous System. 32:61-64 |
| ISSN: | 1433-0350 0256-7040 |
| Popis: | Medulloblastoma (MB) comprises four distinct molecular subgroups, and survival remains particularly poor in patients with Group 3 tumors. Mutations and copy number variations result in altered epigenetic regulation of gene expression in Group 3 MB. Histone deacetylase inhibitors (HDACi) reduce proliferation, promote cell death and neuronal differentiation, and increase sensitivity to radiation and chemotherapy in experimental MB. Bombesin receptor antagonists potentiate the antiproliferative effects of HDACi in lung cancer cells and show promise as experimental therapies for several human cancers. Here, we examined the viability of D283 cells, which belong to Group 3 MB, treated with an HDACi alone or combined with bombesin receptor antagonists.D283 MB cells were treated with different doses of the HDACi sodium butyrate (NaB), the neuromedin B receptor (NMBR) antagonist BIM-23127, the gastrin releasing peptide receptor (GRPR) antagonist RC-3095, or combinations of NaB with each receptor antagonist. Cell viability was examined by cell counting.NaB alone or combined with receptor antagonists reduced cell viability at all doses tested. BIM-23127 alone did not affect cell viability, whereas RC-3095 at an intermediate dose significantly increased cell number.Although HDACi are promising agents to inhibit MB growth, the present results provide preliminary evidence that combining HDACi with bombesin receptor antagonists is not an effective strategy to improve the effects of HDACi against MB cells. |
| Databáze: | OpenAIRE |
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