Polyacrylamide gel electrophoresis of glycoproteins on single concentration and gradient gels
| Autor: | Merwin Moskowitz, B.A. Voyles |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
chemistry.chemical_classification
Glucosamine Chromatography Sodium chemistry.chemical_element Kidney Tritium Biochemistry Genetics and Molecular Biology (miscellaneous) Avian sarcoma virus Cell Line Linear gradient Cell Transformation Neoplastic Avian Sarcoma Viruses chemistry Evaluation Studies as Topic Methods Electrophoresis Polyacrylamide Gel Glycoprotein Polyacrylamide gel electrophoresis Glycoproteins |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Protein Structure. 351:178-181 |
| ISSN: | 0005-2795 |
| DOI: | 10.1016/0005-2795(74)90179-2 |
| Popis: | A comparison of the distribution patterns of glycoproteins released into the medium by normal and oncogenic virus-transformed mammalian cells on 5% and 2–12% linear gradient sodium dodecylsulfate-polyacrylamide gels was made. Marked differences were observed in the distribution patterns obtained with the two types of gels. The gradient gels separate the glycoproteins into three discrete groups, while the 5% gels show only numerous small peaks. It appears that gradient gels may be of unique value in the separation of glycoproteins and may permit the identification of groups of compounds not distinguishable on single porosity gels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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