Inhibition of prostaglandin synthesis leads to a change in adherence of mouse osteoclasts from bone to periosteum
| Autor: | Ian Holt, Michael J. Marshall, Michael W. J. Davie |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism Acid Phosphatase Indomethacin p-Chloromercuribenzoic Acid Down-Regulation Osteoclasts Parathyroid hormone Cysteine Proteinase Inhibitors Dinoprostone Bone resorption Parietal Bone Mice Organ Culture Techniques Endocrinology Osteoclast Periosteum Internal medicine Cell Adhesion medicine Animals Cyclooxygenase Inhibitors Orthopedics and Sports Medicine Incubation Tartrate-resistant acid phosphatase Tartrate-Resistant Acid Phosphatase Chemistry Cell Differentiation Isoenzymes medicine.anatomical_structure Membrane Parathyroid Hormone Calcitonin Mice Inbred CBA Chloromercuribenzoates |
| Zdroj: | Calcified Tissue International. 59:207-213 |
| ISSN: | 1432-0827 0171-967X |
| DOI: | 10.1007/s002239900110 |
| Popis: | When mouse parietal bones were incubated for 1 day in medium containing indomethacin (Ind), the number of tartrate-resistant acid phosphatase-positive osteoclasts (TRAP+OC) counted on the bone surface was drastically reduced. This reduction did not occur with calcitonin or if the endocranial membrane (periosteum) was removed prior to incubation with Ind. The aim of this work was to determine the mechanism involved. TRAP+OC were found to be increased on the endocranial membrane adjacent to the resorbing surface after Ind treatment, compared with cultures supplemented with parathyroid hormone (PTH) or prosta-glandin E2 (PGE2). However, this increase accounted for only half of those lost from the bone surface. TRAP negative osteoclasts were also seen on the membrane and, to a lesser extent, on the bone. Increased TRAP specific activity could be extracted from the endocranial membranes of bones incubated with Ind compared with PGE2 controls. When bones that had been exposed to Ind were then cultured for 1 day in PGE2, an increase in TRAP+OC occurred. This increase was blocked by the removal of the endocranial membrane prior to incubation with PGE2. We conclude that when prostaglandin production ceases, TRAP+OC become less adherent to bone and more adherent to the endocranial membrane. Stimulators of bone resorption appear to reverse this process. |
| Databáze: | OpenAIRE |
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