Recombinant flagellin and its cross-talk with lipopolysaccharide – Effect on pooled chicken peripheral blood mononuclear cells
| Autor: | Shishir Kumar Gupta, Satish S. Gaikwad, C. Madhan Mohan, Rajib Deb, R. Saravanan, Sohini Dey |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
Salmonella typhimurium Nitric Oxide Real-Time Polymerase Chain Reaction Peripheral blood mononuclear cell Microbiology law.invention Immune system law Animals Toll-like receptor General Veterinary biology Interleukin-6 Pattern recognition receptor Receptor Cross-Talk Interleukin-12 Recombinant Proteins TLR5 Leukocytes Mononuclear biology.protein Recombinant DNA TLR4 bacteria Interleukin-4 Chickens Flagellin |
| Zdroj: | Research in Veterinary Science. 95:930-935 |
| ISSN: | 0034-5288 |
| Popis: | Toll-like receptors (TLRs) are one of the types of pattern recognition receptors (PRRs) that recognize conserved pathogen molecules. TLRs link innate and adaptive arms of immune system and are implicated in the development of defense against invading pathogens. Lipopolysaccharide (LPS) and flagellin are recognized by TLR4 and TLR5, respectively. In this study, the effect of flagellin and lipopolysaccharide alone and in combination on chicken peripheral blood mononuclear cells (PBMCs) was investigated. The FliC gene of Salmonella typhimurium was expressed in a prokaryotic expression system and the recombinant flagellin was used to stimulate the chicken PBMCs. A combination of recombinant flagellin and LPS synergistically upregulated nitric oxide production, IL-12 and IL-6 expression but antagonistically down regulated IL-4 expression in comparison to recombinant flagellin alone. The results indicate that these agonists synergistically interact and enhance macrophage function and promote Th1 immune response in chicken PBMCs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect