Local intravascular coagulation and fibrin deposition on intestinal ischemia-reperfusion in rats
| Autor: | Thomas M. van Gulik, Marcel Levi, E. H. Paulina Roossink, Ivo G. Schoots, P.B. Bijlsma |
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| Přispěvatelé: | ACS - Amsterdam Cardiovascular Sciences, AII - Amsterdam institute for Infection and Immunity, General Internal Medicine, Paediatric Gastroenterology, Surgery |
| Rok vydání: | 2003 |
| Předmět: |
Male
Pathology medicine.medical_specialty medicine.medical_treatment Ischemia Fibrinogen Fibrin medicine.artery D-dimer Fibrinolysis medicine Animals Splanchnic Circulation Superior mesenteric artery Rats Wistar Blood Coagulation biology business.industry Microcirculation Water Thrombosis Blood Coagulation Disorders medicine.disease Rats Intestines Glucose Intestinal Absorption Coagulation Reperfusion Injury biology.protein Surgery business Plasminogen activator medicine.drug |
| Zdroj: | Surgery, 133(4), 411-419. Mosby Inc. |
| ISSN: | 0039-6060 |
| DOI: | 10.1067/msy.2003.104 |
| Popis: | Background. This study investigates intravascular coagulation and thrombotic obstruction in the splanchnic vasculature after intestinal ischemia in relation to epithelial integrity and function. Methods. Intestinal ischemia was induced in rats by superior mesenteric artery occlusion for 20 or 40 minutes. Intestinal injury was assessed by histologic analysis, biochemical markers, and functional studies. During reperfusion, portal and systemic blood samples were collected to analyze activation of coagulation and fibrinolysis. Results. Superior mesenteric artery occlusion resulted in mild to moderate intestinal injury. Twenty and 40 minutes of ischemia and 3 hours of reperfusion resulted in local intestinal thrombin generation and conversion of fibrinogen to fibrin, reflected by 3- and 4-fold increases in thrombin-antithrombin complex levels and a 3-fold elevation of fibrin degradation products (D-dimer), respectively. During reperfusion, after a short-lasting initial activation of local fibrinolysis, plasminogen activator activity was suppressed, as indicated by an approximately 4-fold increase in portal plasma levels of the plasminogen activator inhibitor. D-dimer levels showed that activation of coagulation and depression of fibrinolysis resulted in fibrin formation, which was confirmed to be intravascular fibrin deposition by histologic examination. Conclusions. Intestinal ischemia-reperfusion results in local intravascular coagulation and fibrin deposition. (Surgery 2003;133:411-9.) |
| Databáze: | OpenAIRE |
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