Immobilized soybean hull peroxidase for the oxidation of phenolic compounds in coffee processing wastewater
| Autor: | Pricila Maria Batista Chagas, Maria Cristina Silva, Juliana Arriel Torres, Angelita Duarte Corrêa |
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| Rok vydání: | 2015 |
| Předmět: |
Immobilized enzyme
Wastewater Coffee Biochemistry Glutaraldehyde Chitosan Immobilization chemistry.chemical_compound Caffeic Acids Phenols Structural Biology Enzyme Stability Caffeic acid Molecular Biology chemistry.chemical_classification Coffee processing wastewater Chromatography biology Chemistry Temperature food and beverages General Medicine Hydrogen-Ion Concentration Enzymes Immobilized Enzyme assay Microspheres Enzyme Activation Kinetics Enzyme Peroxidases Glutaral biology.protein Soybeans Oxidation-Reduction Peroxidase |
| Zdroj: | International Journal of Biological Macromolecules. 81:568-575 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2015.08.061 |
| Popis: | Chitosan beads were prepared, using glutaraldehyde as a crosslinking agent for the immobilization of soybean hull peroxidase (SBP). The activity of free and immobilized SBP was studied. The optimum pH was 6.0 for both the free and immobilized enzyme; however, enzyme activity became more dependent on the temperature after immobilization. This study evaluated the potential use of immobilized and free enzyme in the oxidation of caffeic acid, of synthetic phenolic solution (SPS) and of total phenolic compounds in coffee processing wastewater (CPW). Some factors, such as reaction time, amount of H2O2 and caffeic acid were evaluated, in order to determine the optimum conditions for enzyme performance. Both enzymes showed a potential in the removal of caffeic acid, SPS and CPW, and immobilized SBP had the highest oxidation performance. The immobilized enzyme showed a potential of 50% in the oxidation of caffeic acid after 4 consecutive cycles. |
| Databáze: | OpenAIRE |
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