Human β-defensin 2 and protease activated receptor-2 expression in patients with chronic periodontitis

Autor: José Roberto Cortelli, Marinella Holzhausen, Sheila Cavalca Cortelli, Gilson Cesar Nobre Franco, Alexandre Lustosa Pereira
Přispěvatelé: Holzhausen, Marinella https://orcid.org/0000-0001-9413-5253, Pereira, Alexandre/C-4227-2016, Holzhausen, Marinella/G-2886-2012, Holzhausen, Marinella/J-4298-2014
Rok vydání: 2012
Předmět:
Male
Pathology
beta-Defensins
Protease activated receptor-2
Diseases
Gastroenterology
Porphyromonas-Gingivalis
Protease-activated receptor 2
biology
Human Beta-Defensin-1
Reverse Transcriptase Polymerase Chain Reaction
Gingival Crevicular Fluid
General Medicine
Middle Aged
Innate Immunity
β defensin 2
Periodontal Treatment
Cytokines
Female
Porphyromonas gingivalis
Human Beta Defensin 2
Adult
Proteases
medicine.medical_specialty
Periodontal treatment
Enzyme-Linked Immunosorbent Assay
Internal medicine
Dentistry
Oral Surgery & Medicine
medicine
Humans
Receptor
PAR-2
Human beta defensin 2
In patient
RNA
Messenger
Saliva
General Dentistry
Aged
Analysis of Variance
Messenger RNA
Chi-Square Distribution
Protease Activated Receptor-2
Dentistry(all)
business.industry
Antimicrobial Peptide Expression
Cell Biology
medicine.disease
biology.organism_classification
Chronic periodontitis
Otorhinolaryngology
Chronic Periodontitis
business
Oral Epithelial-Cells
Zdroj: Web of Science
Repositório Institucional da UNITAU
Universidade de Taubaté (UNITAU)
instacron:UNITAU
ISSN: 0003-9969
DOI: 10.1016/j.archoralbio.2012.04.018
Popis: Made available in DSpace on 2019-09-12T16:53:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objetivo Alguns estudos anteriores mostraram que as gingipains, proteases semelhantes à tripsina produzidas por Porphyromonas gingivalis , regulam positivamente a expressão de mRNA da β defensina-2 humana (HBD-2) por meio do receptor-2 ativado por protease (PAR 2 ) em células epiteliais gengivais. O objetivo deste estudo foi investigar os níveis salivares de HBD-2 e a expressão do mRNA PAR 2 crevicular na periodontite crônica humana e avaliar se o tratamento periodontal afetou esse processo. Métodos Amostras de fluido das fendas gengivais e salivares (GCF) foram coletadas de pacientes periodontais saudáveis ​​(controle) e com periodontite crônica no início do estudo e 50 dias após o tratamento periodontal não cirúrgico. Os níveis salivares de HBD-2 e GCF TNF-α foram analisados ​​por ELISA, e o mRNA de PAR 2 no GCF foi avaliado por RT-PCR. Resultados P. gingivalis foi significativamente ( p < 0,05) mais prevalente em pacientes com periodontite crônica quando comparados aos controles. Essa prevalência diminuiu após a terapia periodontal ( p < 0,0001). O grupo controle apresentou níveis mais baixos estatisticamente significantes de expressão de HBD-2, TNF-α e PAR 2 quando comparado ao grupo com periodontite crônica. Além disso, o tratamento periodontal reduziu significativamente a expressão de PAR 2 e os níveis de HBD-2 em pacientes com periodontite crônica ( p < 0,001). Conclusões Nossos resultados sugerem que os níveis salivares de HBD-2 e a expressão de PAR 2 mRNA do GCF são maiores em indivíduos com periodontite crônica do que em indivíduos saudáveis, e que o tratamento periodontal diminui os níveis de HBD-2 e a expressão de PAR 2 . Objective: Some previous studies have shown that gingipains, trypsin-like proteases produced by Porphyromonas gingivalis, up-regulate human beta defensin-2 (HBD-2) mRNA expression through protease-activated receptor-2 (PAR(2)) in gingival epithelial cells. This study aimed at investigating salivary HBD-2 levels and crevicular PAR(2) mRNA expression in human chronic periodontitis and evaluating whether periodontal treatment affected this process. Methods: Salivary and gingival crevicular fluid (GCF) samples were collected from periodontally healthy (control) and chronic periodontitis patients at baseline and 50 days after nonsurgical periodontal treatment. Salivary HBD-2, and GCF TNF-alpha levels were analysed by ELISA, and PAR(2) mRNA at the GCF was evaluated by RT-PCR. Results: P. gingivalis was significantly (p < 0.05) more prevalent in patients with chronic periodontitis when compared to controls. This prevalence decreased after periodontal therapy (p < 0.0001). The control group showed statistically significant lower levels of HBD-2, TNF-alpha, and PAR(2) expression when compared to the chronic periodontitis group. In addition, periodontal treatment significantly reduced PAR(2) expression and HBD-2 levels in chronic periodontitis patients (p < 0.001). Conclusions: Our results suggest that salivary HBD-2 levels and PAR(2) mRNA expression from GCF are higher in subjects with chronic periodontitis than in healthy subjects, and that periodontal treatment decreases both HBD-2 levels and PAR(2) expression. (C) 2012 Elsevier Ltd. All rights reserved. [Pereira, Alexandre Lustosa; Nobre Franco, Gilson Cesar; Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dept Periodontol [Holzhausen, Marinella] Univ Sao Paulo, Div Periodont, Dept Stomatol, Sch Dent, Sao Paulo, Brazil
Databáze: OpenAIRE
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