Methylated N-(4-N,N-dimethylaminocinnamyl) chitosan-coated electrospray OVA-loaded microparticles for oral vaccination
| Autor: | Praneet Opanasopit, Tanasait Ngawhirunpat, Tasana Pitaksuteepong, Tittaya Suksamran, Warayuth Sajomsang, Theerasak Rojanarata |
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| Rok vydání: | 2013 |
| Předmět: |
Alginates
Cell Survival Ovalbumin Swine medicine.medical_treatment Administration Oral Pharmaceutical Science In Vitro Techniques Methylation Chitosan Mice chemistry.chemical_compound Glucuronic Acid In vivo Oral administration medicine Animals Humans Antigens Intestinal Mucosa Cytotoxicity Mice Inbred BALB C Vaccines Chromatography biology Chemistry Hexuronic Acids Immunogenicity Vaccination Adhesiveness Immunoglobulin A Immunoglobulin G Immunology biology.protein Female Caco-2 Cells Swelling medicine.symptom Adjuvant |
| Zdroj: | International Journal of Pharmaceutics. 448:19-27 |
| ISSN: | 0378-5173 |
| Popis: | The purpose of this study was to prepare microparticles entrapping ovalbumin (OVA) as a model antigen to induce immune responses in mice following oral vaccination. In this study, calcium-alginate and calcium-yam-alginate microparticles were prepared by crosslinking alginate with calcium chloride solution using an electrospraying technique. 0.1% (w/v) of methylated N-(4-N,N-dimethylaminocinnamyl) chitosan (TM65CM50CS) was used to coat microparticles entrapping an initial OVA of 20% w/w to polymer. The results indicated that the coated microparticles were spherical and had a smooth surface, with an average size of 1-3 μm, and were positively charged. In addition, the particles demonstrated a greater swelling and mucoadhesive properties than did uncoated microparticles. The in vitro release from the microparticles indicated that the coated microparticles resulted in more sustained release than uncoated microparticles. The cytotoxicity results showed that all of the formulations were safe. The in vivo oral administration demonstrated that at the same amount of 250 μg OVA, coated microparticles exhibited the highest in vivo adjuvant activity in both IgG and IgA immunogenicity. |
| Databáze: | OpenAIRE |
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