Optimizing the Fluorescence Properties of Nanoemulsions for Single Particle Tracking in Live Cells
| Autor: | Halina Anton, Pichandi Ashokkumar, Tkhe Kyong Fam, Thierry F. Vandamme, Nicolas Anton, Mayeul Collot, Xinyue Wang, Andrey S. Klymchenko |
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| Přispěvatelé: | Conception et application de molécules bioactives (CAMB), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie des Systèmes Fonctionnels, Centre National de la Recherche Scientifique (CNRS), Laboratoire de Bioimagerie et Pathologies (LBP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Biophotonique et Pharmacologie (CNRS UMR 7213), Laboratoire de Biophotonique et Pharmacologie - UMR 7213 (LBP), Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)) |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Polymers Nanoparticle 02 engineering and technology 010402 general chemistry environment and public health 01 natural sciences Fluorescence Polyethylene Glycols chemistry.chemical_compound Pulmonary surfactant Humans General Materials Science Coloring Agents 021001 nanoscience & nanotechnology Biocompatible material Lipids 0104 chemical sciences chemistry Chemical engineering Cell Tracking Single-particle tracking Nanoparticles Emulsions Chimie/Autre lipids (amino acids peptides and proteins) [CHIM.OTHE]Chemical Sciences/Other 0210 nano-technology Ethylene glycol HeLa Cells |
| Zdroj: | ACS Applied Materials & Interfaces ACS Applied Materials & Interfaces, Washington, D.C. : American Chemical Society, 2019, 11 (14), pp.13079-13090. ⟨10.1021/acsami.8b22297⟩ |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | Nanoemulsions (NEs) are biocompatible lipid nanoparticles composed of an oily core stabilized by a surfactant shell. It is acknowledged that the surface decoration with poly(ethylene glycol), through the use of nonionic surfactants, confers high stealth in biological medium with reduced nonspecific interactions. Tracking individual NE by fluorescence microscopy techniques would lead to a better understanding of their behavior in cells and thus require the development of bright single particles with enhanced photostability. However, the understanding of the relationship between the physicochemical properties and chemical composition of the NEs, on the one hand, and its fluorescence properties of encapsulated dyes, on the other hand, remains limited. Herein, we synthesized three new dioxaborine barbituryl styryl (DBS) dyes that displayed high molar extinction coefficients (up to 120 000 M |
| Databáze: | OpenAIRE |
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