Leaf status and environmental signals jointly regulate proline metabolism in winter oilseed rape

Autor: Marie-Françoise Niogret, Sylvain Dechaumet, Alain Bouchereau, Younès Dellero, Nathalie Marnet, Vanessa Clouet, Anthoni Pellizzaro
Přispěvatelé: Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INRA projet 'FLUCOLSA', ANR-11-BTBR-0004,RAPSODYN,Optimisation de la teneur et du rendement en huile chez le colza cultivé sous contrainte azotée(2011), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
Senescence
proline oxidase
Osmotic shock
Proline
Nitrogen
Physiology
Δ1-PYRROLINE-5-CARBOXYLATE SYNTHASE (P5CS)
[SDV]Life Sciences [q-bio]
Brassica
PROLINE DEHYDROGENASE (ProDH)
Plant Science
01 natural sciences
stress osmotique
proline déshydrogénase
03 medical and health sciences
Proline dehydrogenase
Gene Expression Regulation
Plant
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Gene
métabolisme
030304 developmental biology
2. Zero hunger
développement des feuilles
0303 health sciences
sodium chloride stress
ATP synthase
biology
AcademicSubjects/SCI01210
Brassica napus
sénescence foliaire
biology.organism_classification
Research Papers
Plant Leaves
[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding
Biochemistry
Fluxes
Plant—Environment Interactions
biology.protein
osmotic stress
Sink (computing)
010606 plant biology & botany
Regulation
Zdroj: Journal of Experimental Botany
Journal of Experimental Botany, 2020, 71 (6), pp.2098-2111. ⟨10.1093/jxb/erz538⟩
Journal of Experimental Botany, Oxford University Press (OUP), 2020, 71 (6), pp.2098-2111. ⟨10.1093/jxb/erz538⟩
Journal of Experimental Botany, Oxford University Press (OUP), 2019, ⟨10.1093/jxb/erz538⟩
ISSN: 0022-0957
1460-2431
DOI: 10.1093/jxb/erz538⟩
Popis: Illumination conditions and acquisition of source status by winter oilseed rape leaves jointly impact on proline levels, expression levels of proline metabolism-related genes, and associated metabolic fluxes.
Proline metabolism is an essential component of plant adaptation to multiple environmental stress conditions that is also known to participate in specific developmental phases, particularly in reproductive organs. Recent evidence suggested a possible role for proline catabolism in Brassica napus for nitrogen remobilization processes from source leaves at the vegetative stage. Here, we investigate transcript levels of Δ1-PYRROLINE-5-CARBOXYLATE SYNTHASE (P5CS) and PROLINE DEHYDROGENASE (ProDH) genes at the vegetative stage with respect to net proline biosynthesis and degradation fluxes in leaves having a different sink/source balance. We showed that the underexpression of three P5CS1 genes in source leaves was accompanied by a reduced commitment of de novo assimilated 15N towards proline biosynthesis and an overall depletion of free proline content. We found that the expression of ProDH genes was strongly induced by carbon starvation conditions (dark-induced senescence) compared with early senescing leaves. Our results suggested a role for proline catabolism in B. napus, but acting only at a late stage of senescence. In addition, we also identified some P5CS and ProDH genes that were differentially expressed during multiple processes (leaf status, dark to light transition, and stress response).
Databáze: OpenAIRE
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