ACE in three tunicae of rat aorta: expression in smooth muscle and effect of renovascular hypertension
| Autor: | O. Costerousse, T. Battle, M. Challah, François Alhenc-Gelas, Jean-Baptiste Michel, C. Rasetti, Jean-François Arnal, E. Vicaut |
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| Přispěvatelé: | Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpitaux Universitaire Saint-Louis, Lariboisière, Fernand-Widal, this work was supported by Institut National de la Santé et de la Recherche Médicale and by a grant from Brystol-Myers-Squibb Institute for Medical Research. T. Battle was suported by a training grant from Ciba-Geigy Laboratories., The autors thank M. Philippe and F. Savoie for helpful technical assistance and A. Depardieu for artwork |
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Male
Vascular smooth muscle Physiology [SDV]Life Sciences [q-bio] MESH: RNA Messenger / biosynthesis Gene Expression Aorta Thoracic Blood Pressure MESH: Base Sequence Muscle Smooth Vascular Renovascular hypertension MESH: Oligonucleotides Antisense Smooth muscle Reference Values MESH: Animals Carbon Radioisotopes MESH: Carbon Radioisotopes biology MESH: Reference Values MESH: Enalaprilat / pharmacology MESH: Blood Pressure MESH: Muscle Smooth Vascular / enzymology MESH: Peptidyl-Dipeptidase A / metabolism Hypertension Renovascular medicine.anatomical_structure Enalaprilat cardiovascular system MESH: Hypertension Renovascular / enzymology Cardiology and Cardiovascular Medicine MESH: Muscle Smooth Vascular / physiopathology Muscle Contraction MESH: DNA Primers medicine.medical_specialty MESH: Rats Blotting Western Molecular Sequence Data Peptidyl-Dipeptidase A MESH: Aorta Thoracic / drug effects MESH: Muscle Smooth Vascular / drug effects [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system MESH: Muscle Contraction / physiology Physiology (medical) Adventitia Internal medicine medicine.artery Renin–angiotensin system medicine Animals MESH: Blotting Western RNA Messenger Rats Wistar DNA Primers Aorta MESH: Molecular Sequence Data Base Sequence business.industry MESH: Aorta Thoracic / enzymology Angiotensin-converting enzyme MESH: Rats Wistar Oligonucleotides Antisense medicine.disease MESH: Gene Expression MESH: Male Rats MESH: Aorta Thoracic / physiopathology MESH: Muscle Contraction / drug effects Endocrinology biology.protein MESH: Peptidyl-Dipeptidase A / biosynthesis business [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | AJP-Heart and Circulatory Physiology AJP-Heart and Circulatory Physiology, American Physiological Society, 1994, 267 (5), pp.H1777-H1784. ⟨10.1152/ajpheart.1994.267.5.H1777⟩ Scopus-Elsevier |
| ISSN: | 0363-6135 1522-1539 |
| DOI: | 10.1152/ajpheart.1994.267.5.H1777⟩ |
| Popis: | International audience; Angiotensin I-converting enzyme (ACE) is known to be present at the surface of endothelial cells and also in the adventitia in large vessels. The presence of ACE in the vascular smooth muscle remains controversial. We microdissected segments of adventitia and media with or without endothelium from a region devoid of collateral arteries. The membrane-bound ACE activity in the media averaged 41% (pmol [glycine-1-14C]hippuryl-L-histidyl-L-leucine hydrolyzed.g tissue-1.min-1) of the values found in the whole aorta, whereas the adventitia contained only 6%. Immunoreactive ACE in media was characterized by Western blotting. ACE mRNAs were detected and characterized after polymerase chain amplification in isolated media. Angiotensin I and angiotensin II were equally able to contract medial rings, and the response to angiotensin I was blocked by enalaprilat. In aortas of two-kidney, one-clip hypertensive rats, there was an increase in ACE mRNA estimated by ribonuclease protection assay (P = 0.02) and in ACE activity at 15 days and 1 and 3 mo after clipping. This corresponded to a 1.5- to 2-fold increase in the ACE activity of both the media and the adventitia compared with sham-operated rats (P < or = 0.02). Thus ACE gene expression occurs in smooth muscle of rat aorta, which contains roughly the same amount of enzyme as the endothelium and readily converts angiotensin I to angiotensin II. ACE in the medial layer and the adventitia is upregulated in renovascular hypertension. |
| Databáze: | OpenAIRE |
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