Superoxide Dismutase 3 Controls Adaptive Immune Responses and Contributes to the Inhibition of Ovalbumin-Induced Allergic Airway Inflammation in Mice
| Autor: | Kyo-Young Lee, Myung-Ja Kwon, Tae-Yoon Kim, Yun-Jae Jeon |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Ovalbumin
Physiology SOD3 T cell Clinical Biochemistry Adaptive Immunity Biochemistry Mass Spectrometry Superoxide dismutase Mice Immune system Epidermal growth factor Respiratory Hypersensitivity medicine Animals Molecular Biology General Environmental Science Inflammation Mice Knockout biology Superoxide Dismutase Cell Biology Dendritic cell Asthma Mice Inbred C57BL Original Research Communications medicine.anatomical_structure Immunology biology.protein General Earth and Planetary Sciences Transforming growth factor |
| Zdroj: | Antioxidants & Redox Signaling. 17:1376-1392 |
| ISSN: | 1557-7716 1523-0864 |
| DOI: | 10.1089/ars.2012.4572 |
| Popis: | Aims: The extracellular superoxide dismutase 3 (SOD3) is an isoform of SOD. Extensive studies have been focused on role of SOD3 as an antioxidant. However, the role of SOD3 in the immune responses that contribute to the inhibition of allergic lung inflammation has not been investigated. Results: Here, we report for the first time that SOD3 specifically inhibits dendritic cell maturation. Subsequently, SOD3 controls T cell activation and proliferation, and T helper 2 (Th2) and Th17 cell differentiation. As a consequence, the administration of SOD3 into mice alleviated Th2-cell-mediated ovalbumin (OVA)–induced allergic asthma. In addition, we demonstrated that SOD3 inhibits OVA-induced airway extracellular remodeling and Th2 cell trafficking. Through mass spectrometry analysis, the proteins interacting with SOD3 in the lung of asthma were identified. And it was revealed that signaling molecules, such as transforming growth factor (TGF) and epidermal growth factor (EGF) receptor, adhesion and adaptor molecules, kinases, phosphatases, NADPH oxidase, and apoptosis-related factor, were involved, which were altered by administration of SOD3. Relatively severe asthma was observed in SOD3 KO mice and was ameliorated by both the administration of SOD3 and adoptive transfer of SOD3-sufficient CD4 T cells. Moreover, the expression of endogenous SOD3 in the lung peaked early in OVA challenge and gradually decreased upon disease progression, while both SOD1 and SOD2 expression changed relatively little. Innovation and Conclusion: Thus, our data suggest that SOD3 is required to maintain lung homeostasis and acts, at least in part, as a controller of signaling and a decision maker to determine the progression of allergic lung disease. Antioxid. Redox Signal. 17, 1376–1392. |
| Databáze: | OpenAIRE |
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