HPV-ISH-Negative Invasive Cervical Squamous Cell Carcinoma: Histologic and Pap Test Results

Autor: Erika F. Rodriguez, Marissa J. White, Zahra Maleki, Caitlin Alexander
Rok vydání: 2019
Předmět:
Adult
Pathology
medicine.medical_specialty
Histology
Databases
Factual
Cervical Squamous Cell Carcinoma
Biopsy
Population
Uterine Cervical Neoplasms
Atypical Squamous Cells
Human Papillomavirus DNA Tests
Pathology and Forensic Medicine
Surgical pathology
Young Adult
03 medical and health sciences
0302 clinical medicine
Predictive Value of Tests
Cytology
Atypical Squamous Cells of the Cervix
medicine
Humans
Neoplasm Invasiveness
Pap test
education
Papillomaviridae
In Situ Hybridization
Aged
Aged
80 and over
Vaginal Smears
education.field_of_study
medicine.diagnostic_test
business.industry
Reproducibility of Results
General Medicine
Middle Aged
Prognosis
030224 pathology
medicine.disease
stomatognathic diseases
Squamous intraepithelial lesion
030220 oncology & carcinogenesis
Baltimore
DNA
Viral
Carcinoma
Squamous Cell
Female
Squamous Intraepithelial Lesions of the Cervix
business
Papanicolaou Test
Zdroj: Acta Cytologica. 63:417-423
ISSN: 1938-2650
0001-5547
Popis: Introduction: A causal link between infection with a high-risk strain of human papilloma virus (hrHPV) and the development of cervical squamous cell carcinoma (SCC) is well established. However, a small number of SCCs are hrHPV-negative by either HPV co-DNA testing and/or HPV-in situ hybridization (HPV-ISH) at the time of diagnosis. These apparently hrHPV-negative lesions are poorly understood, specifically whether hrHPV-positive precursor lesions exist, which would be detected through hrHPV-based screening. Methods: A search of the pathology archives at the Johns Hopkins Hospital identified women with a diagnosis of hrHPV-negative cervical SCC on surgical specimen. All prior pathologies, including cervical cytology and surgical pathology specimens, and associated hrHPV DNA test results, p16 immunohistochemistry, and HPV-ISH were reviewed. Results: A total of 25 women were identified having a surgical specimen diagnosed as SCC with either negative or equivocal HPV-ISH. Fifteen had a Pap test in the 6 months preceding a diagnosis of SCC, with cytology diagnoses as follows: high-grade squamous intraepithelial lesion n = 14/15; atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion n = 1/15. hrHPV co-testing was performed for 5 of these 15 women and was negative in 2/5 cases. Cervical biopsy was performed for 24 women. HPV-ISH testing, performed on 14 of the biopsy specimens, was negative for 11/14 patients. Of 15 specimens stained for p16, 14 were positive. Conclusion: A subset of patients exist in whom hrHPV is not detectable at or near the time of progression to SCC. Additional research is necessary to further describe this population and determine whether maintaining cytological screening would provide benefit.
Databáze: OpenAIRE
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