| Popis: |
The present study on common carp (Cyprinus carpio L.) sperm was designed to evaluate changes in sperm phenotypic variables during multiple sperm stripping with sperm storage: a) in vivo and b) in vitro. Similar males were multiple injected with carp pituitary (CP) 3 times 3 days apart. Sperm were stored in vivo in the body cavity for 0.5 days (fresh sperm) and 3 days (old sperm) after CP application, then sperm were collected and diluted with a carp extender 1:1 and stored in vitro on ice for 0, 3 and 6 days. In general, fresh sperm from the first stripping had slightly better quality and quantity than old sperm from the second and third stripping, especially in the phenotypic parameters of number of total spermatozoa and number of total motile spermatozoa (P < 0.05). The highest kinetic and quantitative sperm variables were obtained in fresh and old sperm just after sperm collection at 0 days and then they decreased during in vitro sperm storage up to 6 days (P < 0.05). The fertilization, hatching and malformation rates from fresh sperm were similar compared with the old sperm. From the first stripping, fresh sperm, even stored for 6 days in vitro, showed fertility, hatching and malformations at 92.5%, 91.5% and 1.3%, respectively. Multiple hormonal treatments with multiple male stripping together with 0.5 days of in vivo sperm storage followed by 6 days of in vitro storage are methods that can be recommended for use in common carp aquaculture. |
