Nitration of ovalbumin decreases the risk for sensitization via the oral route in a mouse food allergy model
| Autor: | Durga Krishnamurthy, Kathrin Selzle, Susanne C. Diesner, Philipp Starkl, Eva Untersmayr, Elisabeth Förster-Waldl, Ulrich Pöschl, Erika Jensen-Jarolim, Cornelia Schultz, Otto Scheiner, Albert Duschl, Tobias Pfaller, Regina Knittelfelder, Yingyi Zhang, Gertie Janneke Oostingh, Arnold Pollak |
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| Předmět: |
Pulmonary and Respiratory Medicine
Allergy Immunology Immunoglobulin E Epitope 03 medical and health sciences 0302 clinical medicine Immune system Food allergy otorhinolaryngologic diseases Immunology and Allergy Medicine 030223 otorhinolaryngology Sensitization biology business.industry digestive oral and skin physiology RC581-607 medicine.disease 3. Good health Ovalbumin Titer medicine.anatomical_structure 030228 respiratory system biology.protein Oral Presentation Immunologic diseases. Allergy business |
| Zdroj: | Europe PubMed Central Clinical and Translational Allergy Clinical and Translational Allergy, Vol 1, Iss Suppl 1, p O49 (2011) |
| Popis: | Methods and results BALB/c mice were fed untreated (OVA), sham-nitrated (snOVA) or nitrated ovalbumin (nOVA) with or without concomitant acid-suppression. To analyze systemic effects, mice were injected the allergens intraperitoneally (i.p.). Animals being fed OVA or snOVA with antiacids developed elevated IgE, IgG1 and IgG2a titers. Oral immunizations of nOVA under acid-suppression did not result in IgG and IgE formation. However, all i.p. immunized mice revealed high levels of IgE, which were significantly increased in the group being injected nOVA. In RBL-assays, all groups with OVA-specific IgE showed a significant increased mediator release with nOVA as trigger compared to OVA. To analyze the immune response in the involved organ, gastric tissues were screened for cytokine expression by real-time-PCR. Only the acid-suppressed groups being fed OVA or snOVA revealed a higher expression of Th2 and inflammatory markers. In gastric digestion experiments, nOVA was degraded within minutes, whereas OVA and snOVA remained stable up to 120min. Additionally, HPLCchip-MS/MS analysis revealed the most efficiently nitrated tyrosine residue within an ovalbumin epitope recognized exclusively after oral immunization. |
| Databáze: | OpenAIRE |
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