MMP-3-1612 polymorphism - a risk factor for deep venous thrombosis formation
| Autor: | Nai-Xuan Li, La-Mei Yu, Yu-Guo Sheng |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Transfection Risk Assessment law.invention Rats Sprague-Dawley Young Adult Gene Frequency Risk Factors law Animals Humans Medicine Genetic Predisposition to Disease Luciferase Promoter Regions Genetic Allele frequency Genetic Association Studies Polymerase Aged Venous Thrombosis Polymorphism Genetic biology business.industry Promoter Hep G2 Cells Middle Aged medicine.disease Molecular biology In vitro Genotype frequency Disease Models Animal Venous thrombosis Phenotype Case-Control Studies Recombinant DNA biology.protein Female Matrix Metalloproteinase 3 Cardiology and Cardiovascular Medicine business |
| Zdroj: | Vasa. 45:233-239 |
| ISSN: | 1664-2872 0301-1526 |
| DOI: | 10.1024/0301-1526/a000530 |
| Popis: | Abstract. Background: We investigated the association of the 5A/6A polymorphism in the promoter region at -1612 of the matrix metalloproteinase-3 gene (MMP-3-1612) and deep venous thrombosis (DVT). Patients, materials and methods: The distribution of the MMP-3 (-1612 5A/6A) polymorphism in the case and control groups was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum MMP-3 level of two groups was detected using enzyme-linked immunosorbent assay (ELISA). HepG2 cells containing MMP-3-1612 recombinant plasmid were cultured in vitro and the MMP-3 level was defined by luminescence intensity of luciferase. A DVT rat model was built. Serum MMP-3 level in the rats’ wounded vein at different time points was detected by ELISA and recorded for investigation of the association between MMP-3 and DVT. Statistical data analysis was conducted with SPSS18.0. Results: On the basis of the observation of MMP-3-1612 genotype frequency and allele frequency in the case and control groups, we identified significantly higher MMP-3-1612 5A allele frequency and higher serum MMP-3 level in the case group than in the control group (both P < 0.05). According to in vitro luciferase measurements, the 5A allele had higher transcriptional activity than the 6A allele. As observed in the rat model, serum MMP-3 level increased with time passing and thrombosis formation after modelling. Conclusions: The MMP-3-1612 5A/6A polymorphism may effect serum MMP-3 level and over-expression of serum MMP-3 level may be a risk factor for DVT formation. |
| Databáze: | OpenAIRE |
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