Novel immunochromatographic strip assay based on up-conversion nanoparticles for sensitive detection of imidacloprid in agricultural and environmental samples
| Autor: | Zhao Ying, Mingzhou Zhang, Yang Chen, Guonian Zhu, Xinying Lu, Yirong Guo, Rubing Zou, Fangfang Si |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis 010501 environmental sciences 01 natural sciences Rapid detection Chromatography Affinity Neonicotinoids chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry Imidacloprid medicine Environmental Chemistry 0105 earth and related environmental sciences Immunoassay Detection limit Residue (complex analysis) Chromatography medicine.diagnostic_test Pesticide residue Neonicotinoid Agriculture General Medicine Nitro Compounds Pollution Linear range chemistry Nanoparticles Chromatography Liquid |
| Zdroj: | Environmental Science and Pollution Research. 28:49268-49277 |
| ISSN: | 1614-7499 0944-1344 |
| Popis: | Neonicotinoid insecticides are widely used in agriculture for pest control, but the pesticide residues in environmental and agricultural products were a big threat to the health of non-target organisms. In this study, a new immunochromatographic strip test was established for the rapid detection of imidacloprid residue, a neonicotinoid insecticide, based on up-conversion nanoparticles (UCNPs) coupled with the monoclonal antibody against imidacloprid. Under optimal conditions, the half inhibitory concentration (IC50), detection limit, and the linear range of this strip were 8.37 ng/mL, 0.45 ng/mL, and 0.97-250 ng/mL. The strip test could be completed in 30 min. The average recoveries of imidacloprid spiked in water, Chinese cabbages, cucumber, honey, and tea samples were 70.1~101.8%, with coefficient of variations less than 18.9%. The strip was used to test real samples and verified by UPLC-MS/MS method with the good agreement (R2 was 0.9825), indicating this novel strip immunoassay is accurate and reliable. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink