Combining genome composition and differential gene expression analyses reveals that SmPGH1 contributes to bacterial wilt resistance in somatic hybrids
| Autor: | Ting Liu, Jianke Dong, Bingsen Wang, Yan Yu, Xingkui Cai, Haibo Wang, Botao Song, Wenxuan Ye, Zhengnan Cheng, Jun Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Plant Science Hybrid Cells 01 natural sciences Genome Chromosomes Plant Transcriptome 03 medical and health sciences Gene Expression Regulation Plant Solanum melongena Gene Disease Resistance Plant Diseases Plant Proteins Solanum tuberosum Genetics Ralstonia solanacearum biology Bacterial wilt Bulked segregant analysis food and beverages General Medicine biology.organism_classification 030104 developmental biology Gene pool Agronomy and Crop Science Genome Plant 010606 plant biology & botany Microsatellite Repeats |
| Zdroj: | Plant cell reports. 39(9) |
| ISSN: | 1432-203X |
| Popis: | Clarification of the genome composition of the potato + eggplant somatic hybrids cooperated with transcriptome analysis efficiently identified the eggplant gene SmPGH1 that contributes to bacterial wilt resistance. The cultivated potato is susceptible and lacks resistance to bacterial wilt (BW), a soil-borne disease caused by Ralstonia solanacearum. It also has interspecies incompatibility within Solanaceae plants. Previously, we have successfully conducted the protoplast fusion of potato and eggplant and regenerated somatic hybrids that showing resistance to eggplant BW. For efficient use of these novel germplasm and improve BW resistance of cultivated potato, it is essential to dissect the genetic basis of the resistance to BW obtained from eggplant. The strategy of combining genome composition and transcriptome analysis was established to explore the gene that confers BW resistance to the hybrids. Genome composition of the 90 somatic hybrids was studied using genomic in situ hybridization coupled with 44 selected eggplant-specific SSRs (smSSRs). The analysis revealed a diverse set of genome combinations among the hybrids and showed a possibility of integration of alien genes along with the detection of 7 smSSRs linked to BW resistance (BW-linked SSRs) in the hybrids. Transcriptome comparison between the resistant and susceptible gene pools identified a BW resistance associated gene, smPGH1, which was significantly induced by R. solanacearum in the resistant pool. Remarkably, smPGH1 was co-localized with the BW-linked SSR emh01E15 on eggplant chromosome 9, which was further confirmed that smPGH1 was activated by R. solanacearum only in the resistant hybrids. Taken together, the identified gene smPGH1 and BW-linked SSRs have provided novel genetic resources that will aid in potato breeding for BW resistance. |
| Databáze: | OpenAIRE |
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