Changes in functional activities of plasma fibrinogen after treatment with methylene blue and red light
| Autor: | Anna-Maija Suontaka, John Wehrley Chapman, Margareta Blombäck |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Whole Blood Coagulation Time
Light medicine.medical_treatment Immunology Fibrinogen Fibrin chemistry.chemical_compound Nephelometry and Turbidimetry Blood plasma Fibrinolysis medicine Humans Immunology and Allergy Fiber Blood Coagulation Fibrinopeptide A Chromatography Factor XIII biology Chemistry Osmolar Concentration Batroxobin Thrombin Hematology Methylene Blue Blood Clotting time Polymerization Biochemistry biology.protein Virus Inactivation Gels Methylene blue medicine.drug |
| Zdroj: | Transfusion. 43:568-575 |
| ISSN: | 0041-1132 |
| DOI: | 10.1046/j.1537-2995.2003.00377.x |
| Popis: | BACKGROUND : Methylene blue (MB) plus light treatment used for virus inactivation of human plasma units may lead to changes in the functional activities of fibrinogen. STUDY DESIGN AND METHODS : Single-donor units of fresh plasma were treated with 1.0 μ M MB and a red light dose of 48 J per cm 2 . The effects of MB plus red light treatment on fibrinogen clottability, fibrin polymerization and gelation, clot stabilization, and fibrinolysis were studied. RESULTS : The concentration of clottable fibrinogen was unchanged during MB plus red light treatment, but a light-dose-dependent decrease of the concentration of functional fibrinogen was found. The initial release rate of fibrinopeptide A was slightly increased after MB plus red light treatment. Turbidity measurements of fibrin gel showed prolonged clotting time, lower fibrin fiber mass-to-length ratio, and slightly smaller fiber diameter. At a given clotting time, a gel with lower fibrin fiber mass-to-length ratio was produced. Clot stability and fibrinolysis remained normal. l -Histidine added to plasma before MB plus red light treatment normalized the thrombin-induced coagulation time in a dose-dependent way. CONCLUSION : MB plus red light treatment affected the polymerization and gelation phase of fibrin. A tighter fibrin gel structure was formed. No effect on stabilization of fibrin clot or fibrinolysis was found. |
| Databáze: | OpenAIRE |
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