Effects of Moringa Extract on Aminoglycoside-Induced Hair Cell Death and Organ of Corti Damage
| Autor: | Andrew D P Prince, Kathleen C.M. Campbell, Leonard P. Rybak, Michael Brenner, Asmita Dhukhwa, Debashree Mukherjea, Michael T Broderick, Peng Jiang |
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| Rok vydání: | 2021 |
| Předmět: |
Programmed cell death
Apoptosis Mitochondrion Pharmacology Article Moringa Mice Ototoxicity Hair Cells Auditory medicine Animals Organ of Corti Caspase biology Cell Death business.industry Plant Extracts medicine.disease Sensory Systems medicine.anatomical_structure Aminoglycosides Otorhinolaryngology biology.protein Neurology (clinical) Hair cell Gentamicins business |
| Zdroj: | Otol Neurotol |
| ISSN: | 1537-4505 |
| Popis: | Hypothesis Moringa extract, a naturally occurring anti-oxidant, protects against aminoglycoside-induced hair cell death and hearing loss within the organ of Corti. Background Reactive oxygen species (ROS) arise primarily in the mitochondria and have been implicated in aminoglycoside-induced ototoxicity. Mitochondrial dysfunction results in loss of membrane potential, release of caspases, and cell apoptosis. Moringa extract has not previously been examined as a protective agent for aminoglycoside-induced ototoxicity. Methods Putative otoprotective effects of moringa extract were investigated in an organotypic model using murine organ of Corti explants subjected to gentamicin-induced ototoxicity. Assays evaluated hair cell loss, cytochrome oxidase expression, mitochondrial membrane potential integrity, and caspase activity. Results In vitro application of moringa conferred significant protection from gentamicin-induced hair cell loss at dosages from 25 to 300 μg/mL, with dosages above 100 μg/mL conferring near complete protection. Assays demonstrated moringa extract suppression of ROS, preservation of cytochrome oxidase activity, and reduction in caspase production. Conclusion Moringa extract demonstrated potent antioxidant properties with significant protection against gentamicin ototoxicity in cochlear explants. |
| Databáze: | OpenAIRE |
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