Effect of one-week ethanol treatment on monoamine levels and dopaminergic receptors in rat striatum
| Autor: | L.O. Lima, Danielle Silveira Macêdo, F.C.F. Sousa, G.S.B. Viana, Silvânia Maria Mendes Vasconcelos, M.M.F. Fonteles |
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| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Serotonin Physiology Dopamine Immunology Biophysics Kd Rat striatum Biochemistry Norepinephrine (medication) Norepinephrine Internal medicine Dopamine receptor D2 Monoaminergic medicine Animals Biogenic Monoamines General Pharmacology Toxicology and Pharmaceutics Rats Wistar Receptor lcsh:QH301-705.5 Chromatography High Pressure Liquid lcsh:R5-920 Ethanol Chemistry Receptors Dopamine D2 General Neuroscience Receptors Dopamine D1 Central Nervous System Depressants Cell Biology General Medicine Alcohol ingestion Corpus Striatum Rats Endocrinology Monoamine neurotransmitter lcsh:Biology (General) Dopamine receptor Noradrenaline lcsh:Medicine (General) D1 and D2 receptor density medicine.drug |
| Zdroj: | Brazilian Journal of Medical and Biological Research v.36 n.4 2003 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Scopus-Elsevier Brazilian Journal of Medical and Biological Research, Vol 36, Iss 4, Pp 503-509 (2003) Brazilian Journal of Medical and Biological Research, Volume: 36, Issue: 4, Pages: 503-509, Published: APR 2003 |
| ISSN: | 0100-879X |
| Popis: | We studied the effects of ethanol on the levels of norepinephrine, dopamine, serotonin (5-HT) and their metabolites as well as on D1- and D2-like receptors in the rat striatum. Ethanol (2 or 4 g/kg, po) was administered daily by gavage to male Wistar rats and on the 7th day, 30 min or 48 h after drug administration, the striatum was dissected for biochemical assays. Monoamine and metabolite concentrations were measured by HPLC and D1- and D2-like receptor densities were determined by binding assays. Scatchard analyses showed decreases of 30 and 43%, respectively, in D1- and D2-like receptor densities and no change in dissociation constants (Kd) 48 h after the withdrawal of the dose of 4 g/kg. Ethanol, 2 g/kg, was effective only on the density of D2-like receptors but not on Kd of either receptor. Thirty minutes after the last ethanol injection (4 g/kg), decreases of D2 receptor density (45%) as well as of Kd values (34%) were detected. However, there was no significant effect on D1-like receptor density and a 46% decrease was observed in Kd. An increase in dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC), a decrease in norepinephrine, and no alteration in 5-HT levels were demonstrated after 48-h withdrawal of 4 g/kg ethanol. Similar effects were observed in dopamine and DOPAC levels 30 min after drug administration. No alteration in norepinephrine concentration and a decrease in 5-HT levels were seen 30 min after ethanol (4 g/kg) administration. Our findings indicate the involvement of the monoaminergic system in the responses to ethanol. |
| Databáze: | OpenAIRE |
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