Begomovirus Tomato Leaf Curl New Delhi Virus Is Seedborne but Not Seed Transmitted in Melon

Autor: Isabel M. Fortes, Verónica Pérez-Padilla, Beatriz Romero-Rodríguez, Rafael Fernández-Muñoz, Cristina Moyano, Araceli G. Castillo, Leandro De León, Enrique Moriones
Přispěvatelé: Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Romero-Rodríguez, Beatriz, Fernández-Muñoz, Rafael, Castillo, Araceli G., de-León, Leandro, Moriones, Enrique
Rok vydání: 2023
Předmět:
Zdroj: Plant Disease. 107:473-479
ISSN: 1943-7692
0191-2917
DOI: 10.1094/pdis-09-21-1930-re
Popis: Seed transmission can be of considerable relevance to the dissemination of plant viruses in nature and for their prevalence and perpetuation. Long-distance spread of isolates of the begomovirus species Tomato leaf curl New Delhi virus (genus Begomovirus, family Geminiviridae) has recently occurred from Asia to the Middle East and the Mediterranean Basin. Here, we investigated the possible transmission by melon (Cucumis melo L.) seeds of a tomato leaf curl New Delhi virus (ToLCNDV) isolate of the "Spain" strain widely distributed in the Mediterranean area as an alternative mechanism for long-distance spread. PCR amplification detection of ToLCNDV in floral parts and mature seeds of melon plants reveals that this virus is seedborne. "Seedborne" is defined as the ability of a virus to be carried through seeds, which does not necessarily lead to transmission to the next generation. Treatment with a chemical disinfectant significantly reduced the detectable virus associated with melon seeds, suggesting ToLCNDV contamination of the external portion of the seed coat. Also, when the internal fraction of the mature seed (seed cotyledons + embryo) was analyzed by quantitative PCR amplification, ToLCNDV was detectable at low levels, suggesting the potential for viral contamination or infection of the internal portions of seed. However, grow-out studies conducted with melon progeny plants germinated from mature seeds collected from ToLCNDV-infected plants and evaluated at early (1-leaf) or at late (20-leaf) growth stages did not support the transmission of ToLCNDV from seeds to offspring.
This research was partially funded by several grants: grant PID2019-107657RB-C21, funded by the Agencia Estatal de Investigación-Ministerio de Ciencia e Innovación, Spain and P18-RT-1249, from Consejería de Economía, Conocimiento y Universidad, Junta de Andalucía, Spain to E. Moriones; grant PID2019-107657RB-C22, funded by the Agencia Estatal de Investigación-Ministerio de Ciencia e Innovación, Spain to A. G. Castillo; and grant RTI2018-094277-B-C22, funded by the Agencia Estatal de Investigación-Ministerio de Ciencia e Innovación, Spain with the assistance from the European Regional Development Fund, to R. Fernández-Muñoz. We thank S. Fitó for kindly providing melon seeds.
Databáze: OpenAIRE
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