Evidence for mannosidase activities in Streptococcus oralis when grown on glycoproteins as carbohydrate source
| Autor: | Karen A. Homer, H. L. Byers, E. Tarelli, David Beighton |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Mannosidase
Glycan Molecular Sequence Data Ribonuclease B Oligosaccharides medicine.disease_cause Biochemistry Analytical Chemistry Microbiology Substrate Specificity Mannans Ribonucleases Mannosidases medicine Carbohydrate Conformation Chromatography High Pressure Liquid Glycoproteins chemistry.chemical_classification biology Chemistry Streptococcus Organic Chemistry Streptococcus oralis General Medicine Carbohydrate biology.organism_classification Mass spectrometric Carbohydrate Sequence Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Glycoprotein Cell Division |
| Zdroj: | Carbohydrate research. 312(3) |
| ISSN: | 0008-6215 |
| Popis: | Streptococcus oralis when cultured using ribonuclease B as the sole source of carbohydrate, selectively uses the sugars of the Man5 glycoform as shown by HPAEC and MALDI-TOF mass spectrometric analyses. The organism is able to do this by producing novel alpha-(1--3), alpha-(1--6) and beta-(1--4) mannosidase activities and these act in a concerted manner in what appears as a single-step process. The selective utilisation of Man5 is explained by the absence of an alpha-(1--2) mannosidase which is required to initiate breakdown of the glycan chains present in the other glycoforms which are components of the glycoprotein. |
| Databáze: | OpenAIRE |
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