Development of electrospun nanofibers that enable high loading and long-term viability of probiotics
| Autor: | Julijana Kristl, Katja Škrlec, Petra Kocbek, Špela Zupančič, Aleš Berlec, Sonja Prpar Mihevc |
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| Rok vydání: | 2019 |
| Předmět: |
Nanofibers
Pharmaceutical Science 02 engineering and technology 030226 pharmacology & pharmacy law.invention 03 medical and health sciences Probiotic chemistry.chemical_compound fluids and secretions 0302 clinical medicine law Lactobacillus Spectroscopy Fourier Transform Infrared Drug Carriers biology Ethylene oxide Chemistry Probiotics food and beverages High loading General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Trehalose Electrospinning Chemical engineering Nanofiber bacteria 0210 nano-technology Lactobacillus plantarum Biotechnology |
| Zdroj: | European Journal of Pharmaceutics and Biopharmaceutics. 136:108-119 |
| ISSN: | 0939-6411 |
| DOI: | 10.1016/j.ejpb.2019.01.013 |
| Popis: | The interest in probiotics has grown in recent years due to increased awareness of the importance of microbiota for human health. We present the development of monolithic poly(ethylene oxide) and composite poly(ethylene oxide)/lyoprotectant nanofibers loaded with the probiotic Lactobacillus plantarum ATCC 8014. High loading was achieved for L. plantarum cells (up to 7.6 × 108 colony-forming unit/mg) that were either unmodified or expressing mCherry fluorescent protein. The initial concentration of L. plantarum in poly(ethylene oxide) solution was reported, for the first time, as the most critical parameter for its high viability after electrospinning, whereas the applied electric voltage and relative humidity during electrospinning did not vitally impact upon L. plantarum viability. The presence of amorphous lyoprotectant (especially trehalose) in the nanofibers promoted L. plantarum survival due to lyoprotectant interactions with L. plantarum cells. L. plantarum cells in nanofibers were stable over 24 weeks at low temperature, thereby achieving stability comparable with that in lyophilizates. The poly(ethylene oxide) nanofibers released almost all of the L. plantarum cells over 30 min, which will be adequate for their local administration. Our integrated approach enabled development of a promising nanodelivery system that provides high loading and long-term viability of L. plantarum in nanofibers, for local delivery to re-establish the microbiota balance e.g. in vagina. |
| Databáze: | OpenAIRE |
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