Polymorphisms and haplotypes in methylenetetrahydrofolate reductase gene and head and neck squamous cell carcinoma risk
| Autor: | Ana Lívia Silva Galbiatti, Mariangela Torreglosa Ruiz, Eny Maria Goloni-Bertollo, Luiz Sérgio Raposo, José Victor Maniglia, Érika Cristina Pavarino, Juliana Olsen Rodrigues |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Oncology medicine.medical_specialty Alcohol Drinking Genotype Disease Polymerase Chain Reaction Polymorphism Single Nucleotide Sex Factors Internal medicine Odds Ratio Genetics medicine Humans Genetic Predisposition to Disease Allele Molecular Biology Genetic Association Studies Methylenetetrahydrofolate Reductase (NADPH2) DNA Primers biology Smoking Haplotype Head and neck cancer Age Factors General Medicine medicine.disease Head and neck squamous-cell carcinoma Logistic Models Haplotypes Head and Neck Neoplasms Case-Control Studies Methylenetetrahydrofolate reductase DNA methylation Carcinoma Squamous Cell biology.protein Female Brazil Polymorphism Restriction Fragment Length |
| Zdroj: | Molecular Biology Reports. 39:635-643 |
| ISSN: | 1573-4978 0301-4851 |
| Popis: | Functional polymorphisms in genes encoding enzymes involved in folate metabolism might modulate head and neck carcinoma risk because folate participates in DNA methylation and synthesis. We therefore conducted a case–control study of 853 individuals (322 head and neck cancer cases and 531 non-cancer controls) to investigate associations among MTHFR C677T and MTHFR A1298C polymorphisms and head and neck squamous cell carcinoma risk. Interactions between these two polymorphisms and risk factors and clinical histopathological parameters were also evaluated. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to genotype the polymorphisms and Chi-square test and multiple logistic regression were used for statistical analyses. The variables age ≥49 years, male gender, tobacco habits and alcohol consumption, MTHFR 1298 AC or CC genotypes, combined genotypes with two or more polymorphic alleles and 677T and 1298C polymorphic alleles were associated with increased risk for this disease (P |
| Databáze: | OpenAIRE |
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