Flavobacterium pokkalii sp. nov., a novel plant growth promoting native rhizobacteria isolated from pokkali rice grown in coastal saline affected agricultural regions of southern India, Kerala
| Autor: | Tomeu Viver, N. Rameshkumar, Rahul R Menon, Sunitha Kumari |
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| Přispěvatelé: | Department of Science and Technology (India), Council for Scientific and Industrial Research (India), National Institute for Interdisciplinary Science and Technology (India) |
| Rok vydání: | 2020 |
| Předmět: |
DNA
Bacterial India Plant Development Biology Rhizobacteria Microbiology Flavobacterium 03 medical and health sciences Intergenic region Pokkali RNA Ribosomal 16S Phylogeny Soil Microbiology 030304 developmental biology Genetics 0303 health sciences Rhizosphere Phylogenetic tree 030306 microbiology Nucleic Acid Hybridization Brackish rice Oryza equipment and supplies 16S ribosomal RNA biology.organism_classification Housekeeping gene DNA profiling PGPR Multilocus Sequence Typing |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Nine plant-associated bacterial strains designated as L1I52T, NRK F1, NRK F15, NRK F16, NRK F41, NRK F42, NRK F47, NRK F49, and NRK F50 originating from the roots and rhizosphere region of a coastal saline tolerant pokkali rice were taxonomically characterized in this study. Genomic fingerprinting using Enterobacterial Repetitive Intergenic Consensus (ERIC) primers discriminated the nine strains based on the DNA fingerprint patterns indicating that they were not clonal in origin. Phylogenetic analysis using 16S rRNA and other five housekeeping genes (gyrB, glyA, atpA, dnaK and murG) revealed that the novel strains constituted a single novel species within the genus Flavobacterium. In all tree construction methods, the novel strains formed a distinct phylogenetic branch, with Flavobacterium daejeonense GH1-10T, F. sufflavum BBQ-12T, and F. glycines Gm-149T as their nearest phylogenetic neighbours. However, average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) comparison between the draft genomes of L1I52T (representative isolate) and it’s nearest phylogenetic neighbours were well below the proposed threshold values ( This work was carried out with the financial support from Department of Science and Technology (DST), India, as part of ‘startup-research grant for young scientists’ supported project (SB/YS/LS-303/2013) provided to NRK. RRM, SK acknowledges Council of Scientific and Industrial Research (CSIR) for providing research fellowship. CSIR-NIIST manuscript reference number (NIIST/2020/Mar/95). |
| Databáze: | OpenAIRE |
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