Effect of diamond-like carbon doped with chromium on cell differentiation, immune activation and apoptosis
| Autor: | Martina Trávníčková, J Rackova, Marta Vandrovcová, Marie Steinerova, Michal Jelínek, Lucie Bacakova, J Bartova, Margit Zaloudkova, Tomáš Kocourek, Tomas Suchy, Elena Filova |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Diseases of the musculoskeletal system
Cellular differentiation medicine.medical_treatment Osteocalcin lcsh:Surgery osteogenic differentiation Cell Count Core Binding Factor Alpha 1 Subunit Collagen Type I Cell Line Mice biocompatibility Osteogenesis Annexin Cell Adhesion medicine Animals Humans Cell Shape Cell Proliferation biology Chemistry Mesenchymal stem cell apoptosis Cell Differentiation Mesenchymal Stem Cells lcsh:RD1-811 Fibroblasts Alkaline Phosphatase Molecular biology Actins Vinculin macrophages Cytokine Gene Expression Regulation Apoptosis biology.protein Cytokines RNA Alkaline phosphatase Calcium chromium Diamond lcsh:RC925-935 mesenchymal stromal cells Type I collagen |
| Zdroj: | European Cells & Materials, Vol 40, Pp 276-302 (2020) |
| ISSN: | 1473-2262 |
| Popis: | Diamond-like carbon (DLC) is a biocompatible material that has many potential biomedical applications, including in orthopaedics. DLC layers doped with Cr at atomic percent (at.%) of 0, 0.9, 1.8, 7.3, and 7.7 at.% were evaluated with reference to their osteoinductivity with human bone marrow mesenchymal stromal cells (hMSCs), immune activation potential with RAW 264.7 macrophage-like cells, and their effect on apoptosis in Saos-2 human osteoblast-like cells and neonatal human dermal fibroblasts (NHDFs). At mRNA level, hMSCs on DLC doped with 0.9 and 7.7 at.% of Cr reached higher maximum values of both RUNX2 and alkaline phosphatase. An earlier onset of mRNA production of type I collagen and osteocalcin was also observed on these samples; they also supported the production of both type I collagen and osteocalcin. RAW 264.7 macrophages were screened using a RayBio™ Human Cytokine Array for cytokine production. 10 cytokines were at a concentration more than 2 × as high as the concentration of a positive control, but the values for the DLC samples were only moderately higher than the values on glass. NHDF cells, but not Saos-2 cells, had a higher expression of pro-apoptotic markers Bax and Bim and a lower expression of anti-apoptotic factor BCL-XL in proportion to the Cr content. Increased apoptosis was also proven by annexin V staining. These results show that a Cr-doped DLC layer with a lower Cr content can act as an osteoinductive material with relatively low immunogenicity, but that a higher Cr content can induce cell apoptosis. |
| Databáze: | OpenAIRE |
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