Radioimmunological determination of urinary tetrahydroaldosterone
| Autor: | S. Legrand, J.C. Legrand, A. Delassalle, P. Desgrez, A. Lagoguey, A. Carayon, J. Antreassian, Cesselin F |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
medicine.medical_treatment
Microgram Clinical Biochemistry Population Radioimmunoassay Urine Cross Reactions Biochemistry Steroid Excretion Endocrinology Oximes medicine Humans Bovine serum albumin education Aldosterone Molecular Biology Pharmacology Antiserum education.field_of_study Chromatography biology Chemistry Organic Chemistry Hypokalemia biology.protein medicine.symptom |
| Zdroj: | Steroids. 29:725-738 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/0039-128x(77)90117-9 |
| Popis: | A method is presented for radioimmunological determination of 3alpha, 5beta-tetrahydroaldosterone. It is based upon the reactivity of this steroid with an antiserum induced by the 3-carboxymethyloxime of 18, 21-aldosterone diacetate conjugated with bovine serum albumin. One hundred microliters of urine enzymatically hydrolyzed with an helix pomatia preparation, containing tritiated tetrahydroaldosterone for the yield calculation, were extracted with dichloromethane and chromatographed on a small celite column. The yield after extraction and chromatography was 64 +/- 17%. The radioimmunological determination was carried out in a conventional manner. The method is specific, sensitive (10 pg/tube), exact, reproducible, very simple and extremely rapid. The results showed good agreement with values given by a colorimetric method (p less than 0.001). The median value measured in 45 healthy adult subjects under standard sodium diet was 53.3 microgram/24h (95 % of the population within a 16.6 to 131.1 microgram/24h range). In 78 cases of adrenocortical insufficiency, 60 cases of obesity and 28 cases of hypokalemia, the median values (and the ranges : microgram/24h) were respectively 7.7 (1.0 - 51.0), 80.9 (17.0 - 503.0) and 64.3 (8.0 - 181.0). In 330 hypertensive patients the excretion of tetrahydroaldosterone exceeded the normal range in 115 cases (35%) with a median of 199.7 microgram/24h (131 to 620 microgram/24h). |
| Databáze: | OpenAIRE |
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