A Two-Photon-Photocleavable Linker for Triggering Light-Induced Strand Breaks in Oligonucleotides
| Autor: | Xenia M. M. Weyel, Alexander Heckel, Manuela A. H. Fichte |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Oligonucleotides
Electrophoretic Mobility Shift Assay Protein Serine-Threonine Kinases 010402 general chemistry 01 natural sciences Biochemistry Green fluorescent protein chemistry.chemical_compound Drug Delivery Systems Two-photon excitation microscopy Transcription (biology) Coumarins Humans A-DNA Electrophoretic mobility shift assay Chromatography High Pressure Liquid Photons Molecular Structure 010405 organic chemistry Chemistry Oligonucleotide DNA Breaks General Medicine DNA Molecular biology 0104 chemical sciences Biophysics Molecular Medicine Linker |
| Zdroj: | ACS chemical biology. 12(8) |
| ISSN: | 1554-8937 |
| Popis: | We synthesized a two-photon-sensitive photocleavable linker based on the 7-diethylaminocoumarin structure and introduced it successfully into DNA strands. First, we demonstrated the inducibility of strand scissions upon irradiation at 365 nm. To verify and visualize the two-photon activity, we used a fluorescence assay based on a DNA strand displacement immobilized in a hydrogel. Additionally, we investigated its use in a new class of DNA decoys that are able to catch and release nuclear factor κB (NF-κB) by using light as an external trigger signal. In cell culture we were able to show the regulation of NF-κB-controlled transcription of green fluorescent protein. |
| Databáze: | OpenAIRE |
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