Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins
| Autor: | Liguo Wu, Zhenyu Wang, Chengli Hou, Elena Saguer, Dequan Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Antioxidant
purification alcalase Physiology DPPH medicine.medical_treatment Clinical Biochemistry Serum albumin Peptide 01 natural sciences Biochemistry Antioxidants Article Hydrolysate chemistry.chemical_compound 0404 agricultural biotechnology medicine Molecular Biology mass spectrometry chemistry.chemical_classification Chromatography Mass spectrometry biology Blood proteins 010401 analytical chemistry 04 agricultural and veterinary sciences Cell Biology 040401 food science 0104 chemical sciences Amino acid Espectrometria de masses Ultrafiltration (renal) sheep plasma protein antioxidative peptides chemistry Proteïnes de la sang biology.protein Pèptids Peptides |
| Zdroj: | Antioxidants Antioxidants, 2019, vol. 8, núm. 12, p. 592 Articles publicats (D-EQATA) DUGiDocs – Universitat de Girona instname Volume 8 Issue 12 |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox8120592 |
| Popis: | In this study, sheep plasma was submitted to Alcalase-hydrolysis and peptides with better antioxidant properties measured through both the ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability assays were isolated and identified. After hydrolysate ultrafiltration and semi-preparative reverse-phase high-performance liquid chromatography, nine fractions (F1&ndash F9) were obtained, with the two first (F1 and F2) showing the greatest antioxidant potential. These two fractions were further separated by the AKTA purifier system to generate four (F1-1&ndash F1-4) and five (F2-1&ndash F2-5) fractions, respectively, with two of them (F1-2 and F2-1) exhibiting appreciable FRAP activity and DPPH radical scavenging ability. Using liquid chromatography-tandem mass spectrometry, three antioxidant peptides were identified. From their amino acid sequences (QTALVELLK, SLHTLFGDELCK, and MPCTEDYLSLILNR), which include amino acids that have been previously reported as key contributors to the peptide antioxidant properties, it can be maintained that they come mainly from serum albumin. These results suggested that the sheep plasma protein can be considered as a good source of antioxidant peptides and bring forth new possibilities for the utilization of animal blood by-products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|