The effect of cell density on the production of interferon
| Autor: | Dexter S.Y. Seto, Brown Dg, Levy Da, Gerring Jp |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Viral Plaque Assay
Cell division Cell Newcastle disease virus Cell Count Biology Tritium Virus Replication General Biochemistry Genetics and Molecular Biology L Cells (Cell Line) chemistry.chemical_compound Mice L Cells Interferon Leucine Monolayer medicine Animals Carbon Radioisotopes Virology Molecular biology Culture Media Kinetics medicine.anatomical_structure chemistry Viral replication Interferons Thymidine Cell Division medicine.drug |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 145(2) |
| ISSN: | 0037-9727 |
| Popis: | SummaryL-929 cells growing under different conditions of spinner and monolayer cultures were compared according to cell counts, NDV replication, interferon production, and incorporation of radioactive precursors. With equal numbers of cells and NDV replication, the monolayer culture produced 4-32-fold more interferon than that from the respective spinner culture. The spinner and monolayer cultures were also different regarding their metabolic activity as measured by the incorporation of 3H-thymidine and 14C-leucine. DNA and protein synthesis were increased in the spinner culture in contrast to the minimal activity of the monolayer culture. The data shows that the monolayer culture possessed a larger capacity for the induction of interferon than that of the spinner culture, which provides further evidence that the density of cells and their metabolic state influence their production of interferon.We thank Mrs. Jean Simkins and Miss Kathy Lambdin for excellent technical assistance, and Dr. David H. Carver f... |
| Databáze: | OpenAIRE |
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