Bacteroides gingivalis stimulates bone resorption via interleukin-1 production by mononuclear cells The relative role for B. gingivalis endotoxin
| Autor: | Erik Schepens, T. J. Martijn van Steenbergen, Jos W. M. van der Meer, Adri A. Bom-van Noorloos, E.H. Burger, Joke S. van de Gevel |
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| Rok vydání: | 1992 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide medicine.medical_treatment Osteoclasts Peripheral blood mononuclear cell Monocytes Microbiology Mice chemistry.chemical_compound Osteoclast Escherichia coli medicine Animals Bacteroides Humans Pharmacology (medical) Lymphocytes Bone Resorption Pharmacology Lymphokines biology Tumor Necrosis Factor-alpha Chemistry Lymphokine Interleukin biology.organism_classification Molecular biology In vitro Endotoxins Cytokine medicine.anatomical_structure Leukocytes Mononuclear Periodontics Calcium Tumor necrosis factor alpha Interleukin-1 |
| Zdroj: | Journal of Clinical Pharmacy and Therapeutics. 17:409-413 |
| ISSN: | 1365-2710 0269-4727 |
| DOI: | 10.1111/j.1365-2710.1992.tb01209.x |
| Popis: | Supernatants of human peripheral blood mononuclear cells cultured in the presence of B. gingivalis, showed a strong osteoclast stimulating activity as measured by 45Ca release from fetal mouse long bones in vitro. These supernatants also contained a high concentration of bioactive and immunoreactive interleukin-1 (IL-1), but tumor necrosis factor (TNFa), another osteoclast-activating cytokine, was not detected. Osteoclast activation by the supernatants was inhibited by an antibody against IL-1, whereas ultrapure human IL-1 mimicked the effect of the supernatant. The ability of B. gingivalis to induce IL-1 and OAF production was heat sensitive, as 20 min heating of the bacteria at 120 degrees C caused a 50% loss of activity. In addition, purified B. gingivalis lipopolysaccharide (LPS) had little IL-1 inducing capacity, compared with LPS of Escherichia coli. These data suggest that human peripheral blood cells confronted with B. gingivalis produce large amounts of IL-1 which has strong osteoclast stimulating activity. However, in contrast with E. coli LPS, B. gingivalis LPS does not seem to be the major inducing agent. Thus other bacterial components must be responsible for the observed IL-1 and OAF induction. |
| Databáze: | OpenAIRE |
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