Ketamine attenuates osteoarthritis of the knee via modulation of inflammatory responses in a rabbit model
| Autor: | Jing Yao, Wei Lu, Chunxin Wo, Lin Wang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Cancer Research Pathology medicine.medical_specialty Knee Joint ketamine interleukin-10 H&E stain Anti-Inflammatory Agents nuclear factor-κB Enzyme-Linked Immunosorbent Assay Osteoarthritis Biochemistry 03 medical and health sciences 0302 clinical medicine Synovial Fluid Genetics medicine Synovial fluid Animals Ketamine Molecular Biology 030203 arthritis & rheumatology business.industry Cartilage Synovial Membrane NF-kappa B Interleukin Articles medicine.disease Immunohistochemistry Disease Models Animal osteoarthritis 030104 developmental biology medicine.anatomical_structure Oncology Molecular Medicine Cytokines Tumor necrosis factor alpha Rabbits Synovial membrane Inflammation Mediators tumor necrosis factor-α business medicine.drug |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | The aim of the present study was to investigate the efficacy of ketamine in attenuating osteoarthritis (OA) and modulating the expression of inflammatory mediators. A rabbit OA model was established by knee immobilization using plaster bandages. After six weeks, rabbits were randomly allocated into four groups (n=6/group): Normal saline, Ket60, Ket100, and Ket200 and twice a week for four weeks the rabbits received an intra‑articular injection of saline, or 60, 100 or 200 µmol/l ketamine, respectively. One week after the final injection, samples of synovial membrane, synovial fluid and articular cartilage were isolated. The pathological changes were assessed by general observation, hematoxylin and eosin staining and Alcian blue/periodic‑acid Schiff staining. Cartilage pathology was assessed using Mankin's scoring system. Tumor necrosis factor (TNF)‑α and interleukin (IL)‑10 levels in the synovial fluid were measured by enzyme‑linked immunosorbent assays. The nuclear factor (NF)‑κB p65 subunit expression level in cartilage samples was determined by immunohistochemistry. OA was characterized by morpho- logical changes in the articular surface, cartilage lesions, infiltration of inflammatory cells and a significantly increased Mankin's score. Elevated TNF‑α and reduced IL‑10 levels in the synovial fluid , along with increased p65 expression levels in the cartilage were observed in OA rabbits. Intra‑articular injection of ketamine ameliorated the pathological character- istics of OA, reduced the Mankin's score, decreased TNF‑α and NF‑κB p65 expression levels, and increased the level of IL‑10 expression in a dose‑dependent manner. Thus is was demonstrated that Ketamine suppresses the inflammatory response in OA by modulating inflammatory mediator expres - sion levels in a rabbit model of OA. |
| Databáze: | OpenAIRE |
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