Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
| Autor: | Pei-Chi Chung, Hung-Chang Shen, Hung-Hsiang Yu, Tsai-Chi Hsu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Neurons
Lineage (genetic) Cell division General Immunology and Microbiology Mosaicism Neurogenesis Stem Cells General Chemical Engineering General Neuroscience MARCM fungi Mitosis Biology General Biochemistry Genetics and Molecular Biology Cell biology Models Animal Animals Cell Lineage Drosophila Progenitor cell Gene Neural development Developmental biology Developmental Biology |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/55278-v |
| Popis: | Mosaic analysis with a repressible cell marker (MARCM) is a positive mosaic labeling system that has been widely applied in Drosophila neurobiological studies to depict intricate morphologies and to manipulate the function of genes in subsets of neurons within otherwise unmarked and unperturbed organisms. Genetic mosaics generated in the MARCM system are mediated through site-specific recombination between homologous chromosomes within dividing precursor cells to produce both marked (MARCM clones) and unmarked daughter cells during mitosis. An extension of the MARCM method, called twin-spot MARCM (tsMARCM), labels both of the twin cells derived from a common progenitor with two distinct colors. This technique was developed to enable the retrieval of useful information from both hemi-lineages. By comprehensively analyzing different pairs of tsMARCM clones, the tsMARCM system permits high-resolution neural lineage mapping to reveal the exact birth-order of the labeled neurons produced from common progenitor cells. Furthermore, the tsMARCM system also extends gene function studies by permitting the phenotypic analysis of identical neurons of different animals. Here, we describe how to apply the tsMARCM system to facilitate studies of neural development in Drosophila. |
| Databáze: | OpenAIRE |
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