Identification of human T cell leukemia/lymphoma virus type I antibodies, DNA, and protein in patients with polymyositis
| Autor: | Robert Iannone, Pamela Rodgers-Johnson, George H. Collins, Carlos A. Mora, Michael P. Sherman, Clarence J. Gibbs, Rajnikant M. Amin, Owen St. C. Morgan, Lawrence Papsidero, Char G, Bernard J. Poiesz |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty viruses Biopsy Immunology Molecular Sequence Data Retroviridae Proteins Oncogenic Biology Peripheral blood mononuclear cell Polymerase Chain Reaction Virus law.invention Rheumatology immune system diseases law hemic and lymphatic diseases medicine Immunology and Allergy Myocyte Humans Pharmacology (medical) Polymerase chain reaction Muscle biopsy medicine.diagnostic_test Base Sequence Muscles virus diseases Gene Products env Middle Aged Staining HTLV-I Antibodies Polymyositis DNA Viral biology.protein Female Antibody |
| Zdroj: | Arthritis and rheumatism. 38(5) |
| ISSN: | 0004-3591 |
| Popis: | Objective. To investigate a possible association between human T cell leukemia/lymphoma virus type I (HTLV-I) and polymyositis (PM). Methods. Sera and muscle biopsy samples from 9 Jamaican PM patients were compared with specimens from American HTLV-I–positive PM patients and normal controls. Sera were evaluated for HTLV antibodies by enzyme-linked immunosorbent assay and Western blot. The biopsy samples were analyzed for HTLV-I/II DNA by polymerase chain reaction and were also immunohistochemically stained for HTLV gp46 envelope protein. Results. Seven of the 8 Jamaican PM patients from whom sera were available were HTLV-I seropositive. The muscle biopsies of all 9 Jamaican patients demonstrated severe lymphocytic infiltration, cellular degeneration, myofiber atrophy, and fibrosis. Each muscle biopsy specimen contained HTLV-I DNA. Two of 6 samples demonstrated intense staining for HTLV-I gp46 in many of the invading mononuclear cells and weak staining for HTLV-I gp46 in many of the invading mononuclear cells and weak staining in the adjacent myocytes. Two other specimens were weakly positive for gp46 in rare mononuclear cells. All control specimens were negative for the presence of HTLV-I DNA and protein. Conclusion. HTLV-I is associated with an inflammatory muscle disease characterized by direct invasion of the affected muscle by HTLV-I–infected mononuclear cells. |
| Databáze: | OpenAIRE |
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