Antibodies to synthetic peptides for the detection of survivin in tumor tissues
| Autor: | Olga M. Volpina, T. A. Karmakova, E. V. Akhidova, T. D. Volkova, L E Zavalishina, M. P. Filatova, N. M. Vladimirova, Ya. S. Kim, Yu. Yu. Andreeva, D. O. Koroev |
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| Rok vydání: | 2010 |
| Předmět: |
Survivin
Immunoblotting Molecular Sequence Data Breast Neoplasms Endogeny Peptide Biochemistry Antibodies Inhibitor of Apoptosis Proteins law.invention law Biomarkers Tumor medicine Animals Humans Bioorganic chemistry Amino Acid Sequence chemistry.chemical_classification biology Organic Chemistry Cancer medicine.disease Immunohistochemistry Molecular biology Peptide Fragments Recombinant Proteins Urinary Bladder Neoplasms chemistry Recombinant DNA biology.protein Female Rabbits Antibody Microtubule-Associated Proteins Oligopeptides |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1608-330X 1068-1620 |
| Popis: | Survivin, an endogenous protein, is a promising marker for the diagnosis of cancer. The aim of the present work was to obtain antibodies specific to survivin and capable of detecting this protein in tumor tissues. Four peptides corresponding to fragments (1-22), (54-74), (80-88)-(153-165), and (118-144) of the survivin-2B sequence were selected and synthesized. Rabbits were immunized with the synthetic peptides. It has been shown that all peptides in a free state, without conjugation with a high-molecular-weight carrier, stimulate the production of antibodies capable of binding with recombinant survivin. Antipeptide antibodies were isolated from sera and their performance in the immunohistochemical detection of survivin in human tumor tissues was studied. It was shown that only antibodies to the (80-88)-(153-165) peptide bind to the survivin present in breast and bladder tumors. The ability of antibodies to this peptide to detect survivin in tumor tissue lysates was demonstrated by immunoblotting. The part of the sequence targeted by the antibodies against the (80-88)-(153-165) peptide was localized using truncated peptide fragments. |
| Databáze: | OpenAIRE |
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