MicroRNA-138-5p controls sensitivity of nasopharyngeal carcinoma to radiation by targeting EIF4EBP1
| Autor: | Jimmy Yu Wai Chan, Wei Gao, Jacky Wei Kei Lam, Siqi Chen, Raymond K. Y. Tsang, John Zeng Hong Li, Thian-Sze Wong |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.medical_treatment Apoptosis Cell Cycle Proteins Biology Real-Time Polymerase Chain Reaction Radiation Tolerance 03 medical and health sciences 0302 clinical medicine microRNA Autophagy Tumor Cells Cultured otorhinolaryngologic diseases medicine Humans Radiosensitivity Adaptor Proteins Signal Transducing Cell Proliferation Oncogene Cancer Nasopharyngeal Neoplasms General Medicine Cell cycle Phosphoproteins medicine.disease Molecular medicine Radiation therapy MicroRNAs 030104 developmental biology Oncology Nasopharyngeal carcinoma 030220 oncology & carcinogenesis Cancer research DNA Damage |
| Zdroj: | Oncology Reports. 37:913-920 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2017.5354 |
| Popis: | Radiation therapy is the standard treatment for primary nasopharyngeal carcinoma (NPC). MicroRNA regulates cancer responsiveness to radiation therapy by controlling the genes involved in radiation responses. Recent studies suggested that downregulation of microRNA-138-5p was clinically significant in NPC. Here, we evaluated the effect of miR-138-5p on radiosensitivity of NPC cells and explored the underlying mechanisms by identifying its target gene that impacted sensitivity to radiation. Our results revealed that overexpression of miR-138-5p reduced the ability to form colonies, inhibited proliferation, and enhanced radiation-induced DNA damage and autophagy in NPC cells upon radiation treatment. By integrating predicted targets with the transcripts downregulated by miR-138-5p, EIF4EBP1 was identified to be a target gene of miR-138-5p. Results from luciferase reporter assay demonstrated that miR-138-5p downregulated the expression of EIF4EBP1 by binding to the 3'-UTR. Silence of EIF4EBP1 enhanced radiosensitivity of NPC cells as evidenced by reduced ability to form colonies after radiation exposure. In summary, our results indicated that miR-138-5p enhanced radiosensitivity of NPC cells by targeting EIF4EBP1. Further studies are warranted to investigate the potential use of miR-138-5p in the clinical management and treatment prediction of NPC patients. |
| Databáze: | OpenAIRE |
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