Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC–ESI–TOF–MS
| Autor: | Shizhong Chen, Jianhua Sun, Weijuan Jia, Wanwan Wang, Ning Guo, Zongli Bai, Hong Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Rhamnose
monosaccharide hydrolysis Pharmaceutical Science Polysaccharide 01 natural sciences High-performance liquid chromatography Analytical Chemistry lcsh:QD241-441 03 medical and health sciences Hydrolysis chemistry.chemical_compound lcsh:Organic chemistry Glucosamine Drug Discovery Trifluoroacetic acid Monosaccharide Physical and Theoretical Chemistry 030304 developmental biology chemistry.chemical_classification 0303 health sciences composition analysis Chromatography Polyporus umbellatus biology 010401 analytical chemistry Organic Chemistry biology.organism_classification HPLC–MS 0104 chemical sciences HPLC-MS chemistry Chemistry (miscellaneous) polysaccharide Molecular Medicine |
| Zdroj: | Molecules Volume 24 Issue 14 Molecules, Vol 24, Iss 14, p 2526 (2019) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules24142526 |
| Popis: | Polyporus umbellatus is a well-known and important medicinal fungus in Asia. Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects. A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization&ndash ion trap&ndash time of flight&ndash mass spectrometry. The hydrolysis conditions of the polysaccharides were optimized by orthogonal design. The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 ° C and amino sugars 3 mol/L TFA, 3 h, 100 ° C. The resulting monosaccharides derivatized with 1-phenyl-3-methyl-5-pyrazolone have been well separated and analyzed by the established method. Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides. The results showed that polysaccharides in Polyporus umbellatus were composed of mannose, glucosamine, rhamnose, ribose, lyxose, erythrose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and fucose. Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10&ndash 103.70%. This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine. |
| Databáze: | OpenAIRE |
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