Fecal dysbiosis associated with colonic hypersensitivity and behavioral alterations in chronically Blastocystis-infected rats

Autor: Céline Nourrisson, Denis Ardid, Elodie Baudu, Ivan Wawrzyniak, Nicolas Barnich, Amandine Lashermes, Agathe Gelot, Julie Barbier, Virginie Bonnin, Frédéric Delbac, Manon Defaye, Maeva Meynier, Mathieu Meleine, Catherine Godfraind, Benoit Chassaing, Mathilde Bonnet, Philippe Poirier, Frederic A. Carvalho
Přispěvatelé: Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Neuro-Dol (Neuro-Dol), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Laboratoire de Parasitologie et Mycologie [CHU Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Georgia State University, University System of Georgia (USG), Mucosal microbiota in chronic inflammatory diseases [Paris], Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Université Paris Cité (UPCité), Service d'Anatomie Pathologique [CHU Clermont-Ferrand], This work was supported by co-financing from the Region Auvergne-Rhône-Alpes and FEDER in 2015 ('Thématiques émergentes'). Manon Defaye and Elodie Baudu received grants from the Auvergne-Rhône-Alpes Region. This work was supported by the « Ministère de la Recherche et de la Technologie », Inserm and University Clermont Auvergne [UMR1107, UMR1071], INRA [USC-2018], CNRS [UMR6023]., Region Auvergne-Rhone-Alpes European Union (EU) Region Auvergne-Rhone-AlpesRegion Bourgogne-Franche-ComteRegion Hauts-de-FranceRegion Nouvelle-Aquitaine Ministere de la Recherche et de la Technologie Institut National de la Sante et de la Recherche Medicale (Inserm) University Clermont Auvergne UMR1107UMR1071INRA USC-2018Centre National de la Recherche Scientifique (CNRS)UMR6023, Bodescot, Myriam, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Université de Paris (UP), Service d'Anatomopathologie [Clermont-Ferrand], CHU Clermont-Ferrand, Interactions microbiote/mucus dans les maladies inflammatoires chroniques = Mucosal microbiota in chronic inflammatory diseases
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Elevated plus maze
Intestinal parasite
lcsh:Medicine
Blastocystis Infections
Gut flora
medicine.disease_cause
Microbiology
Article
03 medical and health sciences
Colonic Diseases
Feces
0302 clinical medicine
medicine
Animals
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Risk factor
Rats
Wistar
lcsh:Science
Irritable bowel syndrome
Blastocystis
Multidisciplinary
biology
Behavior
Animal
business.industry
Microbiota
lcsh:R
Gastroenterology
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
biology.organism_classification
medicine.disease
Fatty Acids
Volatile
[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
3. Good health
Rats
Disease Models
Animal
030104 developmental biology
ROC Curve
Area Under Curve
Immunology
Dysbiosis
030211 gastroenterology & hepatology
lcsh:Q
Serine Proteases
business
[SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Zdroj: Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
Scientific Reports, 2020, 10 (1), pp.9146. ⟨10.1038/s41598-020-66156-w⟩
Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.9146. ⟨10.1038/s41598-020-66156-w⟩
ISSN: 2045-2322
Popis: Background: Infectious gastroenteritis is a risk factor for the development of post-infectious Irritable Bowel Syndrome (PI-IBS). Recent clinical studies reported a higher prevalence of the intestinal parasite Blastocystis in IBS patients. Using a rat model, we investigated the possible association between Blastocystis infection, colonic hypersensitivity (CHS), behavioral disturbances and gut microbiota changes. Methods: Rats were orally infected with Blastocystis subtype 4 (ST4) cysts, isolated from human stool samples. Colonic sensitivity was assessed by colorectal distension and animal behavior with an automatic behavior recognition system (PhenoTyper), the Elevated Plus Maze test and the Forced Swimming tests. Feces were collected at different time points after infection to study microbiota composition by 16 S rRNA amplicon sequencing and for short-chain fatty acid (SFCA) analysis. Results: Blastocystis-infected animals had non-inflammatory CHS with increased serine protease activity. Infection was also associated with anxiety- and depressive-like behaviors. Analysis of fecal microbiota composition showed an increase in bacterial richness associated with altered microbiota composition. These changes included an increase in the relative abundance of Oscillospira and a decrease in Clostridium, which seem to be associated with lower levels of SCFAs in the feces from infected rats. Conclusions: Our findings suggest that experimental infection of rats with Blastocystis mimics IBS symptoms with the establishment of CHS related to microbiota and metabolic shifts.
Databáze: OpenAIRE
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