Erythropoietin Receptor Signaling Supports Retinal Function after Vascular Injury
| Autor: | Eric Kunz, Aniket Ramshekar, Aaron B. Simmons, Colin A. Bretz, Carson Kennedy, M. Elizabeth Hartnett, Ivan Cardenas |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Genetically modified mouse medicine.medical_specialty Mice Transgenic Biology Retina Article Pathology and Forensic Medicine Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Retinal Diseases Internal medicine Electroretinography Receptors Erythropoietin medicine Animals Outer nuclear layer Erythropoietin Diabetic Retinopathy medicine.diagnostic_test Retinal Vascular System Injuries medicine.disease Erythropoietin receptor 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry 030221 ophthalmology & optometry Female sense organs Signal Transduction medicine.drug Retinopathy |
| Zdroj: | Am J Pathol |
| ISSN: | 0002-9440 |
| DOI: | 10.1016/j.ajpath.2019.11.009 |
| Popis: | The investigation of erythropoietin (EPO) has expanded to include potential nonhematopoietic roles in neural and retinal diseases, including diabetic retinopathy. However, it remains unclear how EPO functions to support the neural retina. Transgenic mice with hypoactive EPO receptor (EPOR) signaling (hWtEPOR) were compared with littermate control mice (WT) to test the role of EPOR signaling under normal conditions and after vascular injury and regrowth into the retina. Although retinal function tested with OptoMotry and electroretinography was comparable to adult (8-week–old) littermate WT mice, hWtEPOR mice had thinner inner and outer plexiform layers and a greater number of amacrine cells. Injury and repair caused by the oxygen-induced retinopathy model reduced visual acuity thresholds, reduced electroretinography amplitudes, and thinned the outer plexiform and inner nuclear layers of both WT and hWtEPOR 8-week–old mice. In hWtEPOR compared with WT mice, scotopic a-wave amplitudes were reduced by injury, despite no change in outer nuclear layer thickness; and peripheral rod, but not cone number, was reduced. Scotopic b-waves were reduced in injured hWtEPOR mice compared with WT, and rod bipolar cell ectopic neurites were increased in both genotypes after injury, suggesting a potential reparative process to preserve connectivity and the b-wave. Normal EPOR signaling appeared important because ectopic neurites and b-waves were lower in the hWtEPOR than WT injured mice. |
| Databáze: | OpenAIRE |
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