Transcription from a gene desert in a melanoma porcine model

Autor: Emmanuelle Bourneuf, Per Wahlberg, Jordi Estellé, Jérôme Lecardonnel, A. Blin, Sylvain Marthey, Francoise Créchet, F. Tessiot, Claire Rogel-Gaillard
Přispěvatelé: Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Acquisition et Analyse de Données pour l'Histoire naturelle (2AD), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de radiobiologie et d'étude du génome (LREG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, Laboratoire Composant pour l'Energie (LCE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), La Ligue Nationale Contre le Cancer (Genetic Epidemiology) PRE09/FD, Institut National du Cancer (INCA) France PL-Bio 5982, French Atomic Energy Commission, INRA (Institut National de la Recherche Agronomique), INRA program 'Jeunes Docteurs', Ligue nationale contre le cancer, ANR-08-GENM-0032,CapSeqAn,Capture de Séquences sur microarrays pour séquençage ciblé de régions d'intérêt : développement sur des études pilotes en génomique Animale(2008), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Rok vydání: 2019
Předmět:
Zdroj: Molecular Genetics and Genomics
Molecular Genetics and Genomics, Springer Verlag, 2020, 295 (5), pp.1239-1252. ⟨10.1007/s00438-020-01694-6⟩
Molecular Genetics and Genomics, 2020, 295 (5), pp.1239-1252. ⟨10.1007/s00438-020-01694-6⟩
ISSN: 1617-4623
1617-4615
Popis: International audience; The genetic mechanisms underlying cutaneous melanoma onset and progression need to be further understood to improve patients' care. Several studies have focused on the genetic determinism of melanoma development in the MeLiM pig, a biomedical model of cutaneous melanoma. The objective of this study was to better describe the influence of a particular genomic region on melanoma progression in the MeliM model. Indeed, a large region of theSus scrofachromosome 1 has been identified by linkage and association analyses, but the causal mechanisms have remained elusive. To deepen the analysis of this candidate region, a dedicated SNP panel was used to fine map the locus, downsizing the interval to less than 2 Mb, in a genomic region located within a large gene desert. Transcription from this locus was addressed using a tiling array strategy and further validated by RT-PCR in a large panel of tissues. Overall, the gene desert showed an extensive transcriptional landscape, notably dominated by repeated element transcription in tumor and fetal tissues. The transcription of LINE-1 and PERVs has been confirmed in skin and tumor samples from MeLiM pigs. In conclusion, although this study still does not identify a candidate mutation for melanoma occurrence or progression, it highlights a potential role of repeated element transcriptional activity in the MeLiM model.
Databáze: OpenAIRE
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