Comparison of three enzyme-linked immunosorbent assays for serologic diagnosis of contagious agalactia in sheep
| Autor: | Michel Aubert, Thierry Rotis, D. Bergonier, Philippe Dufour, Anne Van de Wiele, Michel Pépin, Aurèle Valognes, Maurice Lambert |
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| Přispěvatelé: | Inconnu, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
0301 basic medicine
Time Factors 040301 veterinary sciences Mycoplasma agalactiae [SDV]Life Sciences [q-bio] 030106 microbiology ved/biology.organism_classification_rank.species Sheep Diseases Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Sensitivity and Specificity 0403 veterinary science 03 medical and health sciences Mycoplasma medicine Animals Mycoplasma Infections Sheep Domestic ComputingMilieux_MISCELLANEOUS 2. Zero hunger Kappa value General Veterinary Serologic diagnosis ved/biology 04 agricultural and veterinary sciences Virology [SDV] Life Sciences [q-bio] Antibody response True negative Female Flock Reagent Kits Diagnostic Blood sampling |
| Zdroj: | Journal of Veterinary Diagnostic Investigation Journal of Veterinary Diagnostic Investigation, American Association of Veterinary Laboratory Diagnosticians, 2003, 15 (3), pp.281-285 Scopus-Elsevier |
| ISSN: | 1040-6387 |
| Popis: | Serologic diagnosis of ovine contagious agalactia ( Mycoplasma agalactiae) with the enzyme-linked immunosorbent assay (ELISA) developed by Agence Française de Séceurité Sanitaire des Aliments (AFS-SA) may produce a few false-positive (FP) and false-negative (FN) results. When the prevalence of disease is low, these erroneous results may generate problems for eradication schemes. To prevent this, 2 commercial ELISAs were compared with the AFSSA ELISA. Flocks of known status were selected and classified into 4 categories: true positive (TP), FP, true negative (TN), and FN; 20 sheep per flock were submitted for blood sampling. A flock was considered positive when at least 1 out of 20 sera was positive or 2 sera were doubtful. In the flock, the diagnostic sensitivity of the 3 kits was very good (100%), and the diagnostic specificity showed an improvement from 46% (AFSSA test) to 88% and 92% (commercial tests). Considering individual animals, very few positive ewes were detected within TN or FP flocks; the proportion of positive ewes varied greatly from one kit to another (48% to 82%) within TP flocks. The kinetics of antibody response in sheep experimentally infected with various field strains of M. agalactiae were quite similar with all 3 ELISAs. The agreement between the 3 tests, assessed using the kappa value, varied from moderate to good (respective values of 0.56, 0.61, and 0.86). The 2 commercial ELISAs showed better performances, probably because of a superior analytical sensitivity, and are a good alternative for the serodiagnosis of contagious agalactia in sheep. |
| Databáze: | OpenAIRE |
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