Poxviruses package viral redox proteins in lateral bodies and modulate the host oxidative response
| Autor: | Karel Novy, David Albrecht, Abigail Collopy, Jason Mercer, Melanie Krause, Jemima J. Burden, Susanna R. Bidgood, Bernd Wollscheid |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
viruses
Immunology Vaccinia virus Virions Viral core Oxidation-reduction reactions Transcription factors Viral replication Respiratory infections Fractionation Oxidative phosphorylation Biology medicine.disease_cause Virus Replication Microbiology Virus Cell Line chemistry.chemical_compound Viral Proteins Viral entry Virology Gene expression Genetics medicine Molecular Biology Effector Poxviridae virus diseases Cell biology chemistry Proteome Parasitology Vaccinia Oxidation-Reduction Oxidative stress |
| Zdroj: | PLoS Pathogens, 18 (7) |
| ISSN: | 1553-7374 1553-7366 |
| Popis: | All poxviruses contain a set of proteinaceous structures termed lateral bodies (LB) that deliver viral effector proteins into the host cytosol during virus entry. To date, the spatial proteotype of LBs remains unknown. Using the prototypic poxvirus, vaccinia virus (VACV), we employed a quantitative comparative mass spectrometry strategy to determine the poxvirus LB proteome. We identified a large population of candidate cellular proteins, the majority being mitochondrial, and 15 candidate viral LB proteins. Strikingly, one-third of these are VACV redox proteins whose LB residency could be confirmed using super-resolution microscopy. We show that VACV infection exerts an anti-oxidative effect on host cells and that artificial induction of oxidative stress impacts early and late gene expression as well as virion production. Using targeted repression and/or deletion viruses we found that deletion of individual LB-redox proteins was insufficient for host redox modulation suggesting there may be functional redundancy. In addition to defining the spatial proteotype of VACV LBs, these findings implicate poxvirus redox proteins as potential modulators of host oxidative anti-viral responses and provide a solid starting point for future investigations into the role of LB resident proteins in host immunomodulation. PLoS Pathogens, 18 (7) ISSN:1553-7374 ISSN:1553-7366 |
| Databáze: | OpenAIRE |
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