In vitro analysis of the trajectories of adhesive microbubbles approaching endothelial cells
| Autor: | Francesco Brasili, Flavia Righi Riva, Gaio Paradossi, Barbara Cerroni, Yosra Toumia, Letizia Oddo, Fabio Domenici, Elisabetta Tortorella |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
microbubbles
adhesion rolling targeting rgd integrins Endothelium Contrast Media 02 engineering and technology 010402 general chemistry 01 natural sciences Umbilical vein Biomaterials Colloid and Surface Chemistry Settore CHIM/02 Adhesives medicine Humans Ultrasonography Microbubbles Adhesion Rolling Targeting RGD Integrins Chemistry Endothelial Cells Adhesion 021001 nanoscience & nanotechnology In vitro 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Coupling (electronics) medicine.anatomical_structure Biophysics Microbubbles Adhesive 0210 nano-technology Preclinical imaging |
| Zdroj: | Journal of Colloid and Interface Science. 578:758-767 |
| ISSN: | 0021-9797 |
| DOI: | 10.1016/j.jcis.2020.06.009 |
| Popis: | Adhesion is a key process when ultrasound contrast agents, i.e. microbubbles, approach pathological tissues. A way to accomplish tumour targeting is to tether surface engineered microbubbles to endothelial cells of the up-regulated vascularization of cancer tissues. This can be achieved by coupling the microbubbles surface with the Arginine-Glycine-Aspartate, RGD, sequence. Such molecule interacts with the integrin receptors placed on the endothelial cells. Stability and trajectories of RGD modified lipid shelled MBs have been analysed in vitro using microchannels coated with human umbilical vein endothelial cells, HUVEC. In the microchannels realistic conditions, close to the physiological ones, were reproduced replicating shear rate, roughness comparable to the endothelium and channel size mimicking the postcapillary venules. In these conditions, the analysis of the trajectories close to the walls highlights a substantial difference between the modified MBs and the plain ones. Moreover, MBs adhesion has dynamic features recalling the motion of neutrophils engaged near the substrate such as rolling, translations and transient detachments. These findings are useful for the optimization of in vivo imaging and targeting functions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect