Turtles of the generaGeoemydaandPangshura(Testudines: Geoemydidae) lack differentiated sex chromosomes: the end of a 40-year error cascade forPangshura
| Autor: | Tomáš Protiva, Petr Velenský, Michail Rovatsos, Peter Praschag, Markus Auer, Lukáš Kratochvíl, Uwe Fritz, Lorenzo Clemente, Sofia Mazzoleni, Barbora Augstenová |
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| Rok vydání: | 2019 |
| Předmět: |
ZW sex-determination system
Evolution Karyotype Comparative genome hybridization Geoemyda spengleri lcsh:Medicine Geoemydidae General Biochemistry Genetics and Molecular Biology FISH Constitutive heterochromatin Pangshura biology General Neuroscience lcsh:R Microsatellite Sex chromosomes General Medicine Sex determination biology.organism_classification Evolutionary Studies Turtles Geoemyda Telomeres Evolutionary biology General Agricultural and Biological Sciences Zoology |
| Zdroj: | PeerJ PeerJ, Vol 7, p e6241 (2019) |
| ISSN: | 2167-8359 |
| DOI: | 10.7717/peerj.6241 |
| Popis: | For a long time, turtles of the family Geoemydidae have been considered exceptional because representatives of this family were thought to possess a wide variety of sex determination systems. In the present study, we cytogenetically studiedGeoemyda spengleriandG. japonicaand re-examined the putative presence of sex chromosomes inPangshura smithii. Karyotypes were examined by assessing the occurrence of constitutive heterochromatin, by comparative genome hybridization andin situhybridization with repetitive motifs, which are often accumulated on differentiated sex chromosomes in reptiles. We found similar karyotypes, similar distributions of constitutive heterochromatin and a similar topology of tested repetitive motifs for all three species. We did not detect differentiated sex chromosomes in any of the species. ForP. smithii, a ZZ/ZW sex determination system, with differentiated sex chromosomes, was described more than 40 years ago, but this finding has never been re-examined and was cited in all reviews of sex determination in reptiles. Here, we show that the identification of sex chromosomes in the original report was based on the erroneous pairing of chromosomes in the karyogram, causing over decades an error cascade regarding the inferences derived from the putative existence of female heterogamety in geoemydid turtles. |
| Databáze: | OpenAIRE |
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