Hydraulic pressure inducing renal tubular epithelial-myofibroblast transdifferentiation in vitro
| Autor: | Jun-ming Fan, Zi Li, Rong Zheng, Xi-sheng Xie, Jiang Wu, Fei-yan Li |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Cellular differentiation Biology General Biochemistry Genetics and Molecular Biology Cell Line Myoblasts Internal medicine Pressure medicine Animals Myocyte General Pharmacology Toxicology and Pharmaceutics Cytoskeleton Actin General Veterinary Transdifferentiation Cell Differentiation Epithelial Cells General Medicine Fibroblasts Rats Cell biology Biomedicine Kidney Tubules Endocrinology Cytoplasm Cell Transdifferentiation Myofibroblast |
| Zdroj: | Journal of Zhejiang University SCIENCE B. 10:659-667 |
| ISSN: | 1862-1783 1673-1581 |
| DOI: | 10.1631/jzus.b0920110 |
| Popis: | Objective: The effects of hydraulic pressure on renal tubular epithelial-myofibroblast transdifferentiation (TEMT) were investigated. Methods: We applied hydraulic pressure (50 cmH2O) to normal rat kidney tubular epithelial cells (NRK52E) for different durations. Furthermore, different pressure magnitudes were applied to cells. The morphology, cytoskeleton, and expression of myofibroblastic marker protein and transforming growth factor-β1 (TGF-β1) of NRK52E cells were examined. Results: Disorganized actin filaments and formation of curling clusters in actin were seen in the cytoplasm of pressurized cells. We verified that de novo expression of α-smooth muscle actin induced by pressure, which indicated TEMT, was dependent on both the magnitude and duration of pressure. TGF-β1 expression was significantly upregulated under certain conditions, which implies that the induction of TEMT by hydraulic pressure is related with TGF-β1. Conclusion: We illustrate for the first time that hydraulic pressure can induce TEMT in a pressure magnitude- and duration-dependent manner, and that this TEMT is accompanied by TGF-β1 secretion. |
| Databáze: | OpenAIRE |
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